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In-depth quantitative analysis and comparison of the human hepatocyte and hepatoma cell line HepG2 proteomes
Max Planck Inst Biochem, Biochem Prote Grp, Dept Prote & Signal Transduct, Klopferspitz 18, D-82152 Martinsried, Germany..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Upper Abdominal Surgery.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy. Uppsala University, Science for Life Laboratory, SciLifeLab.
2016 (English)In: Journal of Proteomics, ISSN 1874-3919, E-ISSN 1876-7737, Vol. 136, 234-247 p.Article in journal (Refereed) PublishedText
Abstract [en]

Hepatocytes play a pivotal role in human homeostasis. They are essential in regulation of glucose and lipid levels in blood and play a central role in metabolism of amino acids, lipids, drugs and xenobiotic-compounds. In addition, hepatocytes produce a major portion of proteins circulating in the blood. Hepatocytes were isolated from liver tissue obtained from surgical resections. Proteins were extracted and processed using filter aided sample preparation protocol and were analyzed by LC-MS/MS using high accuracy mass spectrometry. Proteins were quantified by the 'Total Protein Approach' and 'Proteomic Ruler'. We report a comprehensive proteomic analysis of purified human hepatocytes and the human hepatoma cell line HepG2. The complete dataset comprises 9400 proteins and provides a comprehensive and quantitative depiction of the proteomes of hepatocytes and HepG2 cells at the protein titer and copy number dimensions. We describe basic cell organization and in detail energy metabolism pathways and metabolite transport. We provide quantitative insights into protein synthesis and drug and xenobiotics catabolism. Our data delineate differences between the native human hepatocytes and HepG2 cells by providing for the first time quantitative data at protein concentrations and copy numbers. 

Place, publisher, year, edition, pages
2016. Vol. 136, 234-247 p.
Keyword [en]
Human hepatocyte, Human hepatocyte proteome, Quantitative proteomics, Metabolism, Transporter, ADME proteins, 'Proteomic Ruler', Total Protein Approach', FASP
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-292987DOI: 10.1016/j.jprot.2016.01.016ISI: 000372692600023OAI: oai:DiVA.org:uu-292987DiVA: diva2:927281
Max Planck SocietySwedish Research Council, 2822
Available from: 2016-05-11 Created: 2016-05-11 Last updated: 2016-05-11Bibliographically approved

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Vildhede, AnnaNorén, AgnetaArtursson, Per
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