uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid
Show others and affiliations
2016 (English)In: Nanomedicine: Nanotechnology, Biology and Medicine, ISSN 1549-9634, E-ISSN 1549-9642, Vol. 12, no 4, 1033-1043 p.Article in journal (Refereed) PublishedText
Abstract [en]

UNLABELLED: When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate semi-quantitative profiles of corona proteins formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.; FROM THE CLINICAL EDITOR: Inhaled nanoparticles often acquire a layer of protein corona while they go through the respiratory tract. Here, the authors investigated the identity of these proteins. The proper identification would improve the understanding of the use of inhaled nanoparticles in future therapeutics.

Place, publisher, year, edition, pages
2016. Vol. 12, no 4, 1033-1043 p.
Keyword [en]
Protein corona; Nanoparticle; Respiratory tract lining fluid; Plasma; Silica; Poly(vinyl) acetate; Proteomics
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-293056DOI: 10.1016/j.nano.2015.12.369ISI: 000375577300017PubMedID: 26767511OAI: oai:DiVA.org:uu-293056DiVA: diva2:927306
Funder
EU, European Research CouncilSwedish Heart Lung FoundationGlaxoSmithKline (GSK)
Available from: 2016-05-11 Created: 2016-05-11 Last updated: 2016-06-30Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Eriksson, JonnyEdwards, Katarina
By organisation
Analytical Chemistry
In the same journal
Nanomedicine: Nanotechnology, Biology and Medicine
Pharmaceutical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 98 hits
ReferencesLink to record
Permanent link

Direct link