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Growth factor analysis of low-grade glioma CSF: PDGF and VEGF are not detectable
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Neurology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Ludwiginstitutet för Cancerforskning.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Neurology.
2003 (English)In: Neurological Sciences, ISSN 1590-1874, E-ISSN 1590-3478, Vol. 24, no 2, 70-73 p.Article in journal (Refereed) Published
Abstract [en]

Platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF-2) are involved in the development of grade 2 gliomas. The aim of the present study was to determine the presence of these growth factors in the cerebrospinal fluid (CSF) and to assess their usefulness as biological markers. CSF was collected from 7 adult patients with newly diagnosed supratentorial low-grade gliomas by lumbar puncture and was analysed together with matched serum samples using radioreceptor and enzyme-linked immunosorbant assays. Neither PDGF nor VEGF were detected in the CSF, and FGF-2 was measurable at extremely low concentrations in only 2 of 7 patients. Serum levels were within normal limits. We conclude that these growth factors are not released into the CSF in any significant amounts and are therefore not suitable as biological markers in grade 2 gliomas.

Place, publisher, year, edition, pages
2003. Vol. 24, no 2, 70-73 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-64954PubMedID: 12827542OAI: oai:DiVA.org:uu-64954DiVA: diva2:92865
Available from: 2007-04-25 Created: 2007-04-25 Last updated: 2017-11-30Bibliographically approved
In thesis
1. In Search of Prognostic Factors in Grade 2 Gliomas
Open this publication in new window or tab >>In Search of Prognostic Factors in Grade 2 Gliomas
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Grade 2 gliomas are malignant brain tumours affecting otherwise healthy adults. Although the long-term prognosis is poor, many patients are well and may have a high quality of life for several years. There is, however, a large variability in the natural course of the disease which makes it essential to identify patients who might benefit from early surgery or radio-therapy. The aim of the present thesis was to define new and clinically useful prognostic markers that may assist in the initial treatment decision and in patient follow-up.

A retrospective study of 189 patients with gliomas WHO grade 2 showed no advantage in survival of early tumour resection or radiotherapy, and confirmed that histological subtype and patient age are the most important predictors of survival (I). In 89 patients, the pre-treatment uptake of 11C-methionine (MET) measured with positron emission tomography (PET) was identified as a prognostic marker for survival (II). At the time of tumour progression, irradiated tumours demonstrated signs of a residual radiotherapeutic effect that correlated with the pre-treatment uptake of MET (III). Pre-treatment uptake of MET may, therefore, be important both in predicting the natural course of the disease and the response after treatment. Immunohistochemical staining of 40 tumour samples showed an inverse association between the number of tumour cells expressing platelet-derived growth factor alpha receptor (PDGFRa) and survival (IV). Also, a reduction was observed in the number of receptor-positive cells after malignant transformation, supporting the prognostic value of PDGFRa.

Lumbar puncture was performed in eight patients with newly diagnosed low-grade gliomas to identify three important growth factors in tumour development. Neither PDGF nor vascular endothelial growth factor (VEGF) were detected in the cerebrospinal fluid (CSF), and fibroblast growth factor 2 (FGF-2) was measurable at extremely low concentrations in two of the patients (V). A proteome screening of the CSF, using two-dimensional gel electrophoresis and mass spectrometry, detected alpha 2-HS glycoprotein at significantly higher concentrations than in a control group (VI). This glycoprotein emerges as a novel substance in glioma research and may be of great interest because of its suggested involvement in the embryonic development of the neocortex.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2002. 39 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1194
Keyword
Neurosciences, low-grade glioma, positron emission tomography, platelet-derived growth factor receptor, mass spectrometry, alpha 2-HS glycoprotein, Neurovetenskap
National Category
Neurology
Research subject
Neurology
Identifiers
urn:nbn:se:uu:diva-2789 (URN)91-554-5435-6 (ISBN)
Public defence
2002-12-14, Rudbecksalen, Uppsala, 13:15
Opponent
Available from: 2002-10-25 Created: 2002-10-25 Last updated: 2013-05-23Bibliographically approved

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Larsson, AndersSmits, Anja

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