uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Transcriptional regulation of human oxysterol 7alpha-hydroxylase by sterol response element binding protein
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. (Pharm Biochemistry)
2004 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 316, no 1, 158-164 p.Article in journal (Refereed) Published
Abstract [en]

Oxysterol 7alpha-hydroxylase (CYP7B1) metabolizes oxysterols, potent regulators of lipid homeostasis. Very little is known about transcriptional regulation of human CYP7B1. The present results indicate that sterol response element binding protein (SREBP), a family of oxysterol-responsive transcription factors that stimulates cholesterol synthesis, may be an important regulator of CYP7B1. SREBP suppressed a human CYP7B1 luciferase reporter gene in several cell lines, most markedly in rat hepatoma McA-RH7777 cells. An SREBP-1-responsive region was mapped to a GC-rich sequence in the proximal CYP7B1 promoter, containing binding sites for the basal transcriptional activator Sp1. Mutagenesis of this sequence abolished SREBP-1-mediated suppression. Data indicated that SREBP does not bind this sequence but affects the gene indirectly, probably via interaction with Sp1. Our findings indicate that CYP7B1 transcription is controlled by SREBP and reveal a link between oxysterol-sensitive regulators and oxysterol metabolism. We propose that CYP7B1 is important for regulating cellular sterol content and protects against oxysterol-mediated toxicity.

Place, publisher, year, edition, pages
2004. Vol. 316, no 1, 158-164 p.
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-65198DOI: 10.1016/j.bbrc.2004.02.029PubMedID: 15003524OAI: oai:DiVA.org:uu-65198DiVA: diva2:93109
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-03-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Norlin, Maria
By organisation
Department of Pharmaceutical Biosciences
In the same journal
Biochemical and Biophysical Research Communications - BBRC
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 273 hits
ReferencesLink to record
Permanent link

Direct link