Isolation of high purity 1-[2′,4′-dihydroxy-3′,5′-di-(3″-methylbut-2″-enyl)-6′-methoxy] phenylethanone from Acronychia pedunculata (L.) Miq. by high-speed counter-current chromatography
2004 (English)In: Journal of Chromatography A, ISSN 0021-9673, Vol. 1022, no 1-2, 213-216 p.Article in journal (Refereed) Published
Following an initial clean-up step on silica, high-speed counter-current chromatography (HSCCC) was used to purify an aryl ketone, 1-[2′,4′-dihydroxy-3′,5′-di-(3″-methylbut-2″-enyl)-6′-methoxy] phenylethanone from an extract of the stem bark of the shrub Acronychia pedunculata. The two-phase solvent system used was composed of n-heptane–ethyl acetate–methanol–water at an optimized volume ratio of 4:1:4:1 (v/v/v/v). Target compound (58.1 mg) with a purity of 98.9% was obtained after HSCCC of 183.5 mg sample with a purity of 35.7% recovered after the silica clean-up step. Identification of the target compound was performed by 1H NMR, 13C NMR, two-dimensional NMR and LC–electrospray ionization MS.
Place, publisher, year, edition, pages
2004. Vol. 1022, no 1-2, 213-216 p.
Acronychia pedunculata, Counter-current chromatography, Pharmaceutical analysis, Dihydroxydimethylbutenylmethoxyl phenylethanone
IdentifiersURN: urn:nbn:se:uu:diva-65225DOI: 10.1016/j.chroma.2003.09.046OAI: oai:DiVA.org:uu-65225DiVA: diva2:93136