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Differential effects of three echovirus strains on cell lysis and insulin secretion in beta cell derived lines
Lund Univ, Skane Univ Hosp, Dept Clin Sci, Cellular Autoimmun Unit, Jan Waldenstroms Gata 35, SE-20502 Malmo, Sweden..
Lund Univ, Skane Univ Hosp, Dept Clin Sci, Unit Diabet & Celiac Dis, Jan Waldenstroms Gata 35, SE-20502 Malmo, Sweden..
Lund Univ, Skane Univ Hosp, Dept Clin Sci, Cellular Autoimmun Unit, Jan Waldenstroms Gata 35, SE-20502 Malmo, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
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2016 (English)In: Journal of Medical Virology, ISSN 0146-6615, E-ISSN 1096-9071, Vol. 88, no 6, 971-978 p.Article in journal (Refereed) PublishedText
Abstract [en]

In an earlier study, infection of human pancreatic islets with epidemic strains of echovirus (E4, E16, E30), with proven but differently ability to induce islet autoimmunity, resulted either in a severe damage (i.e., E16 and E30) or proceeded without visible changes in infected islets (i.e., E4). In this study, the ability of these strains to replicate in beta cells and the consequence of such an infection for beta cell lysis and beta cell function was studied in the pancreatic beta cell lines INS-1, MIN6, and NIT-1. The strains of E16 and E30 did replicate in INS1, MIN6, and NIT1 cells and resulted in a pronounced cytopathic effect within 3 days following infection. By contrast, E4 replicated in all examined insulinoma cells with no apparent cell destruction. The insulin release in response to high glucose stimulation was hampered in all infected cells (P<0.05) when no evidence of cytolysis was present; however, the adverse effect of E16 and E30 on insulin secretion appeared to be higher than that of the E4 strain. The differential effects of echovirus infection on cell lysis, and beta cell function in the rodent insulinoma INS1, MIN6, and NIT 1 cells reflect those previously obtained in primary human islets and support the notion that the insulinproducing beta cells can harbor a non-cytopathic viral infection.

Place, publisher, year, edition, pages
2016. Vol. 88, no 6, 971-978 p.
Keyword [en]
enterovirus, type 1 diabetes, MIN 6, INS 1, NIT 1
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-294641DOI: 10.1002/jmv.24438ISI: 000373627000007PubMedID: 26629879OAI: oai:DiVA.org:uu-294641DiVA: diva2:932538
Funder
Swedish Child Diabetes FoundationThe Crafoord FoundationSwedish Society for Medical Research (SSMF)
Available from: 2016-06-01 Created: 2016-05-26 Last updated: 2016-06-01Bibliographically approved

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Anagandula, Mahesh
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Clinical ImmunologyDepartment of Immunology, Genetics and Pathology
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