Potential of Synechocystis PCC 6803 as a novel cyanobacterial chassis for heterologous expression of enzymes in the trans-resveratrol biosynthetic pathway
2016 (English)In: Protein Expression and Purification, ISSN 1046-5928, E-ISSN 1096-0279, Vol. 121, 163-168 p.Article in journal (Refereed) PublishedText
Selected model strains of phototrophic cyanobacteria have been genetically engineered for heterologous expression of numerous enzymes. In the present study, we initially explored the heterologous expression of enzymes involved in trans-resveratrol production, namely, the production of tyrosine ammonia-lyase, coumaroyl CoA-ligase, and stilbene synthase, in the unicellular cyanobacterium Synechocystis PCC 6803. Under the promoters Ptrc1Ocore and Ptrc1O, the respective genes were transcribed and translated into the corresponding soluble proteins at concentrations of 16-34 mu g L-1. The expression levels of these enzymes did not affect the growth rate of the cyanobacterial cells. Interestingly, coumaroyl CoA-ligase expression slightly increased the chlorophyll a content of the cells. Overall, our results suggest that the complete pathway of trans-resveratrol production can be engineered in Synechocystis PCC 6803.
Place, publisher, year, edition, pages
2016. Vol. 121, 163-168 p.
Cyanobacteria, Heterologous expression, Synechocystis PCC 6803, Trans-resveratrol
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-294654DOI: 10.1016/j.pep.2016.01.020ISI: 000373662300023PubMedID: 26845578OAI: oai:DiVA.org:uu-294654DiVA: diva2:932715
FunderSwedish Energy AgencyKnut and Alice Wallenberg Foundation