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Potential of Synechocystis PCC 6803 as a novel cyanobacterial chassis for heterologous expression of enzymes in the trans-resveratrol biosynthetic pathway
Chulalongkorn Univ, Program Biotechnol, Fac Sci, Bangkok 10330, Thailand.;Chulalongkorn Univ, Dept Biochem, Fac Sci, Bangkok 10330, Thailand..
Chulalongkorn Univ, Dept Biochem, Fac Sci, Bangkok 10330, Thailand..
Chulalongkorn Univ, Dept Biochem, Fac Sci, Bangkok 10330, Thailand.;Chulalongkorn Univ, Omics Sci & Bioinformat Ctr, Bangkok 10330, Thailand..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. Uppsala University, Science for Life Laboratory, SciLifeLab.
2016 (English)In: Protein Expression and Purification, ISSN 1046-5928, E-ISSN 1096-0279, Vol. 121, p. 163-168Article in journal (Refereed) Published
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Abstract [en]

Selected model strains of phototrophic cyanobacteria have been genetically engineered for heterologous expression of numerous enzymes. In the present study, we initially explored the heterologous expression of enzymes involved in trans-resveratrol production, namely, the production of tyrosine ammonia-lyase, coumaroyl CoA-ligase, and stilbene synthase, in the unicellular cyanobacterium Synechocystis PCC 6803. Under the promoters Ptrc1Ocore and Ptrc1O, the respective genes were transcribed and translated into the corresponding soluble proteins at concentrations of 16-34 mu g L-1. The expression levels of these enzymes did not affect the growth rate of the cyanobacterial cells. Interestingly, coumaroyl CoA-ligase expression slightly increased the chlorophyll a content of the cells. Overall, our results suggest that the complete pathway of trans-resveratrol production can be engineered in Synechocystis PCC 6803.

Place, publisher, year, edition, pages
2016. Vol. 121, p. 163-168
Keyword [en]
Cyanobacteria, Heterologous expression, Synechocystis PCC 6803, Trans-resveratrol
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-294654DOI: 10.1016/j.pep.2016.01.020ISI: 000373662300023PubMedID: 26845578OAI: oai:DiVA.org:uu-294654DiVA, id: diva2:932715
Funder
Swedish Energy AgencyKnut and Alice Wallenberg Foundation
Available from: 2016-06-02 Created: 2016-05-26 Last updated: 2017-11-30Bibliographically approved

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Lindblad, Peter

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