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Engineered light controlled cell development for enhanced hydrogen production in Nostoc punctiforme ATCC 29133
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Microbial Chemistry. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2016 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

The aim of this thesis is to enhance heterocyst-based hydrogen production inNostoc punctiforme ATCC 29133. We envision to do so by finely regulatingthe ratio of heterocyst in order to optimize the filament energy balance. Wehereby report the development of an optogenetic synthetic switch basedon the native PcpeC promoter. The optogenetic switch featured a 24-folddynamic range when measuring reporter sfGFP fluorescence. Such a geneticgate was conceived to artificially drive the expression of hetR, the masterregulator of heterocyst development. We achieved to induce enhancedheterocyst differentiation in the presence of ammonia only by changing thechromatic properties of the light source. Thus, the natural cell developmentregulation was substituted by effectively introducing a full person-drivencontrol over the process.

Place, publisher, year, edition, pages
2016. , 70 p.
National Category
Renewable Bioenergy Research
Identifiers
URN: urn:nbn:se:uu:diva-294854OAI: oai:DiVA.org:uu-294854DiVA: diva2:933497
Presentation
2016-02-11, 14:15 (English)
Supervisors
Available from: 2016-06-08 Created: 2016-05-28 Last updated: 2016-06-08Bibliographically approved

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