uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Homogeneous detection of single rolling circle replication products
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
2004 (English)In: Analytical Chemistry, ISSN 0003-2700, Vol. 76, no 2, 495-8 p.Article in journal (Refereed) Published
Abstract [en]

We describe a simple and straightforward approach for homogeneous and isothermal detection of individual rolling circle replication (RCR) products, which represent individual padlock probe circularization events. The RCR products constitute tens of kilobases long single-stranded tandem repeated copies of the probe sequence, and in solution, they fold into micrometer-sized random coils. The method is based on the local enrichment of fluorescence-labeled probes that hybridize to the coiled RCR products compared to the concentration of free probes in solution. We present a detailed characterization of the fluorescence-labeled products using a highly sensitive and fast microscopy setup. At a 10(4)-fold excess of free label, we were able to detect and follow individual RCR products at a signal-to-background noise ratio of 27. This high signal-to-background noise ratio leaves room for analysis in a simple detection device at higher speeds or at lower labeling ratios.

Place, publisher, year, edition, pages
2004. Vol. 76, no 2, 495-8 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-65446DOI: 10.1021/ac034987+PubMedID: 14719904 OAI: oai:DiVA.org:uu-65446DiVA: diva2:93357
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2009-04-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nilsson, Mats
By organisation
Department of Genetics and Pathology
In the same journal
Analytical Chemistry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 261 hits
ReferencesLink to record
Permanent link

Direct link