uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Difference in the induction, but not in the repair, of X-ray- and nitrogen ion-induced DNA single-strand breaks as measured using human cell extracts
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences. (BMS)
2003 (English)In: International Journal of Radiation Biology, ISSN 0955-3002, Vol. 79, no 12, 965-71 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE: To compare the repair efficiency of X-ray (low linear energy transfer [LET]) and nitrogen ion (high LET)-induced single-strand breaks (SSB) in a human cell-free end-joining system. MATERIALS AND METHODS: SSB were introduced into a bacterial plasmid, pBR322, by X-rays (4 MeV photons) and nitrogen ions with an LET=125 keV micro m(-1). Repair efficiency was studied under incubation with the protein extracts from human squamous carcinoma cells, UT-SCC-5. RESULTS: A several fold higher dose of nitrogen ion radiation compared with X-ray radiation was needed to induce a similar loss of supercoiled plasmid DNA. There was no difference in the repair efficiency of SSB induced by these two types of radiation. CONCLUSION: The data indicate that X-rays at 25 Gy and nitroging ions at 100 Gy radiation doses, under condition of low scavenging capacity (10 mM Tris), induce SSB of similar complexity or, alternatively, differences in SSB complexity do not alter the repair rate.

Place, publisher, year, edition, pages
2003. Vol. 79, no 12, 965-71 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-65489DOI: 10.1080/09553000310001632912PubMedID: 14713574OAI: oai:DiVA.org:uu-65489DiVA: diva2:93400
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2009-07-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Biomedical Radiation Sciences
In the same journal
International Journal of Radiation Biology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 124 hits
ReferencesLink to record
Permanent link

Direct link