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Interaction of PEG-ylated Lipid Nanoparticles with Silica Substrates
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC.
2016 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

In this project, the interaction between polyethylene glycol modified (PEG-ylated) lipid nanoparticles and silica substrates was studied to find out how this interaction was affected by bulk concentration, temperature and the composition of particles. One kind of lipodisk and four kinds of PEG-ylated liposome were prepared from lipid films and characterized by quartz crystal microbalance with dissipation monitoring (QCM-D) instrument mounted with silica sensor. The detailed information of particle-silica interaction could be obtained from the raw data, frequency and dissipation values, and the adsorbed mass surface density calculated from the raw data. Lipodisks could be immobilized on the silica surface. Whether they would be rinsed away by PBS buffer was influenced by both the bulk concentration and temperature. The way of their binding could change and the changing process was affected by temperature. PEG-ylated liposomes could also be immobilized on the silica surface, and they could break and spread to form supported lipid bilayer in certain conditions, for example, the changing of temperature or the using of certain lipids. Supported lipid bilayers were created with high reproducibility in this project, which could be very useful to the future study of transmembrane proteins functions and lipodisk properties.

Place, publisher, year, edition, pages
2016. , 26 p.
Series
UPKEM C, 86
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-296349OAI: oai:DiVA.org:uu-296349DiVA: diva2:937607
Subject / course
Chemistry
Educational program
Bachelor Programme in Chemistry
Presentation
2016-05-30, 11:15 (English)
Supervisors
Examiners
Available from: 2016-06-15 Created: 2016-06-15 Last updated: 2016-06-15Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2017-06-15 18:00
Available from 2017-06-15 18:00

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Department of Chemistry - BMC
Chemical Sciences

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