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Trafficking of Endogenous Immunoglobulins by Endothelial Cells at the Blood-Brain Barrier
Roche Innovat Ctr Basel, Roche Pharma Res & Early Dev pRED, Neurodegenerat & Regenerat, Basel, Switzerland..
Roche Innovat Ctr Basel, Roche Pharma Res & Early Dev pRED, Neurodegenerat & Regenerat, Basel, Switzerland..
UdS, Coll France, CNRS, IGBMC,INSERM,ICS, BP 10142, Strasbourg, France..
Roche Innovat Ctr Basel, Roche Pharma Res & Early Dev pRED, Neurodegenerat & Regenerat, Basel, Switzerland..
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2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 25658Article in journal (Refereed) Published
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Abstract [en]

The Blood-Brain Barrier (BBB) restricts access of large molecules to the brain. The low endocytic activity of brain endothelial cells (BECs) is believed to limit delivery of immunoglobulins (IgG) to the brain parenchyma. Here, we report that endogenous mouse IgG are localized within intracellular vesicles at steady state in BECs in vivo. Using high-resolution quantitative microscopy, we found a fraction of endocytosed IgG in lysosomes. We observed that loss of pericytes (key components of the BBB) in pdgf-b(ret/ret) mice affects the intracellular distribution of endogenous mouse IgG in BECs. In these mice, endogenous IgG was not detected within lysosomes but instead accumulate at the basement membrane and brain parenchyma. Such IgG accumulation could be due to reduced lysosomal clearance and increased sorting to the abluminal membrane of BECs. Our results suggest that, in addition to low uptake from circulation, IgG lysosomal degradation may be a downstream mechanism by which BECs further restrict IgG access to the brain.

Place, publisher, year, edition, pages
2016. Vol. 6, 25658
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-297270DOI: 10.1038/srep25658ISI: 000375436400001PubMedID: 27149947OAI: oai:DiVA.org:uu-297270DiVA: diva2:941992
Available from: 2016-06-23 Created: 2016-06-22 Last updated: 2017-11-28Bibliographically approved

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Betsholtz, Christer

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