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Promoter regulation: designing cells for biotechnological applications
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. (Karin Stensjös grupp, Molekylär biomimetik; Mikrobiell kemi)
2016 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The filamentous cyanobacteria Nostoc punctiforme ATCC 29133 is a model species fordevelopment of sustainable production methods of numerous compounds. One of its uniquefeatures is the anaerobic environment of the strains nitrogen fixing heterocyst cells. To be ableto properly utilize this environment, more knowledge regarding what regulates cell specificexpression is required. In this study, three motifs of the NsiR I promoter of Anabaena sp.PCC 7120 was studied in this system utilizing YFP-fluorescence as a reporter to determinetheir impact on spatial expression pattern. Investigations were performed on immobilizedcells with the use of confocal microscopy and results point towards sigma factor regulation.

Place, publisher, year, edition, pages
2016. , 66 p.
UPTEC X, 16 021
Keyword [en]
Cyanobacteria, heterocyst, promoter, specificity, confocal microscopy, immobilization
National Category
URN: urn:nbn:se:uu:diva-297502OAI: oai:DiVA.org:uu-297502DiVA: diva2:942126
Educational program
Molecular Biotechnology Engineering Programme
2016-06-03, C2:305, BMC, Husargatan 3, Uppsala, 15:15 (English)
Available from: 2016-07-01 Created: 2016-06-23 Last updated: 2016-07-01Bibliographically approved

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