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Hyaluronic Acid Hydrogels Formed in Situ by Transglutaminase-Catalyzed Reaction
Ecole Polytech Fed Lausanne, Sch Life Sci, Inst Bioengn, Lab Stem Cell Bioengn, CH-1015 Lausanne, Switzerland.;Ecole Polytech Fed Lausanne, Sch Engn, CH-1015 Lausanne, Switzerland.;Katholieke Univ Leuven, Dept Mech Engn, Biomech Sect, Celestijnenlaan 300, B-3001 Leuven, Belgium..
Ecole Polytech Fed Lausanne, Sch Life Sci, Inst Bioengn, Lab Stem Cell Bioengn, CH-1015 Lausanne, Switzerland.;Ecole Polytech Fed Lausanne, Sch Engn, CH-1015 Lausanne, Switzerland.;Ecole Polytech Fed Lausanne, Sch Basic Sci, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
2016 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 17, no 5, 1553-1560 p.Article in journal (Refereed) PublishedText
Abstract [en]

Enzymatically cross-linked hydrogels can be formed in situ and permit highly versatile and selective tethering of bioactive molecules, thereby allowing for a wealth of applications in cell biology and tissue engineering. While a number of studies have reported the bioconjugation of extracellular matrix (ECM) proteins and peptides into such matrices, the site specific incorporation of biologically highly relevant polysaccharides such as hyaluronic acid (HA) has thus far not been reported, limiting our ability to reconstruct this key feature of the in vivo ECM. Here we demonstrate a novel strategy for transglutaminase-mediated covalent linking of HA moieties to a synthetic poly(ethylene glycol) (PEG) macromer resulting in the formation of hybrid HA-PEG hydrogels. We characterize the ensuing matrix properties and demonstrate how these cytocompatible gels can serve to modulate the cellular phenotype of human mammary cancer epithelial cells as well as mouse myoblasts. The use of HA as a novel building block in the increasingly varied library of synthetic PEG-based artificial ECMs should have applications as a structural as well as a signaling component and offers significant potential as an injectable matrix for regenerative medicine.

Place, publisher, year, edition, pages
2016. Vol. 17, no 5, 1553-1560 p.
National Category
Biochemistry and Molecular Biology Polymer Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-297802DOI: 10.1021/acs.biomac.5b01587ISI: 000375886500001PubMedID: 27014785OAI: oai:DiVA.org:uu-297802DiVA: diva2:943505
Funder
EU, FP7, Seventh Framework Programme
Available from: 2016-06-28 Created: 2016-06-28 Last updated: 2016-06-28Bibliographically approved

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Hilborn, JönsOssipov, Dmitri A.
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