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Electron tomography reveals the fibril structure and lipid interactions in amyloid deposits
Univ Ulm, Inst Prot Biochem, D-89081 Ulm, Germany..
Univ Ulm, Inst Prot Biochem, D-89081 Ulm, Germany..
Univ Ulm, Inst Prot Biochem, D-89081 Ulm, Germany..
Univ Ulm, Inst Prot Biochem, D-89081 Ulm, Germany..
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2016 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 113, no 20, 5604-5609 p.Article in journal (Refereed) PublishedText
Abstract [en]

Electron tomography is an increasingly powerful method to study the detailed architecture of macromolecular complexes or cellular structures. Applied to amyloid deposits formed in a cell culture model of systemic amyloid A amyloidosis, we could determine the structural morphology of the fibrils directly in the deposit. The deposited fibrils are arranged in different networks, and depending on the relative fibril orientation, we can distinguish between fibril meshworks, fibril bundles, and amyloid stars. These networks are frequently infiltrated by vesicular lipid inclusions that may originate from the death of the amyloid-forming cells. Our data support the role of nonfibril components for constructing fibril deposits and provide structural views of different types of lipid-fibril interactions.

Place, publisher, year, edition, pages
2016. Vol. 113, no 20, 5604-5609 p.
Keyword [en]
aggregation, conformational disease, electron tomography, protein assembly, prion
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-297779DOI: 10.1073/pnas.1523496113ISI: 000375977600044PubMedID: 27140609OAI: oai:DiVA.org:uu-297779DiVA: diva2:943634
Funder
German Research Foundation (DFG), FA 456/15-1German Research Foundation (DFG), HA 7138/2-1Swedish Research Council
Available from: 2016-06-28 Created: 2016-06-28 Last updated: 2016-06-28Bibliographically approved

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Westermark, PerWestermark, Gunilla T.
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Department of Immunology, Genetics and PathologyDepartment of Medical Cell Biology
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Proceedings of the National Academy of Sciences of the United States of America
Cell and Molecular Biology

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