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Modulation of the enzymatic activities of replicative helicase (DnaB) by interaction with Hp0897: a possible mechanism for helicase loading in Helicobacter pylori
Jawaharlal Nehru Univ, Special Ctr Mol Med, New Delhi 110067, India.;Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India..
Jawaharlal Nehru Univ, Special Ctr Mol Med, New Delhi 110067, India..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. Jawaharlal Nehru Univ, Special Ctr Mol Med, New Delhi 110067, India..
Natl Inst Cholera & Enter Dis, Kolkata 700010, India..
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2016 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 44, no 7, 3288-3303 p.Article in journal (Refereed) PublishedText
Abstract [en]

DNA replication in Helicobacter pylori is initiated from a unique site (oriC) on its chromosome where several proteins assemble to form a functional replisome. The assembly of H. pylori replication machinery is similar to that of the model gram negative bacterium Escherichia coli except for the absence of DnaC needed to recruit the hexameric DnaB helicase at the replisome assembly site. In the absence of an obvious DnaC homologue in H. pylori, the question arises as to whether HpDnaB helicase is loaded at the Hp-replication origin by itself or is assisted by other unidentified protein(s). A high-throughput yeast two-hybrid study has revealed two proteins of unknown functions (Hp0897 and Hp0340) that interact with HpDnaB. Here we demonstrate that Hp0897 interacts with HpDnaB helicase in vitro as well as in vivo. Furthermore, the interaction stimulates the DNA binding activity of HpDnaB and modulates its adenosine triphosphate hydrolysis and helicase activities significantly. Prior complex formation of Hp0897 and HpDnaB enhances the binding/loading of DnaB onto DNA. Hp0897, along with HpDnaB, colocalizes with replication complex at initiation but does not move with the replisome during elongation. Together, these results suggest a possible role of Hp0897 in loading of HpDnaB at oriC.

Place, publisher, year, edition, pages
2016. Vol. 44, no 7, 3288-3303 p.
National Category
Cell and Molecular Biology
URN: urn:nbn:se:uu:diva-297903DOI: 10.1093/nar/gkw148ISI: 000375800200035PubMedID: 27001508OAI: oai:DiVA.org:uu-297903DiVA: diva2:944353
Available from: 2016-06-29 Created: 2016-06-28 Last updated: 2016-06-29Bibliographically approved

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