Heparin interactions with apoA1 and SAA in inflammation-associated HDL
2016 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 474, no 2, 309-314 p.Article in journal (Refereed) PublishedText
Apolipoprotein A1 (apoA1) is the main protein component responsible for transportation of cholesterol on high-density lipoprotein (HDL). Serum amyloid A (SAA) is an acute phase protein associated with HDL. Apart from their physiological functions, both apoA1 and SAA have been identified as 'amyloidogenic peptides'. We report herein that the polysaccharide heparin interacts with both apoA1 and SAA in HDL isolated from plasma of inflamed mice. The reaction is rapid, forming complex aggregates composed of heparin, apoA1 and SAA as revealed by gel electrophoresis. This interaction is dependent on the size and concentration of added heparin. Mass spectrometry analysis of peptides derived from chemically crosslinked HDL-SAA particles detected multiple crosslinks between apoA1 and SAA, indicating close proximity (within 25 angstrom) of these two proteins on the HDL surface, providing a molecular and structural mechanism for the simultaneous binding of heparin to apoA1 and SAA.
Place, publisher, year, edition, pages
2016. Vol. 474, no 2, 309-314 p.
Heparan sulfate, Lipoproteins/receptors, Amyloid, Atherosclerosis, Proteoglycans, Mass spectrometry
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-298081DOI: 10.1016/j.bbrc.2016.04.092ISI: 000376221700013PubMedID: 27105909OAI: oai:DiVA.org:uu-298081DiVA: diva2:944792
FunderSwedish Research Council, K2012-67X-21128-04-4Swedish Heart Lung Foundation, 20140131