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Inhalation anesthesia of rats: influence of the fraction of inspired oxygen on limb ischemia/reperfusion injury
Univ Bern, Dept Clin Res, Murtenstr 50,POB 44, CH-3008 Bern, Switzerland.;Univ Bern, Grad Sch Cellular & Biomed Sci, Murtenstr 50,POB 44, CH-3008 Bern, Switzerland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Univ Zurich Hosp, Div Plast Surg & Hand Surg, CH-8091 Zurich, Switzerland..
Univ Bern, Dept Clin Res, Murtenstr 50,POB 44, CH-3008 Bern, Switzerland..
2016 (English)In: Laboratory Animals. Journal of the Laboratory Animal Science Association, ISSN 0023-6772, E-ISSN 1758-1117, Vol. 50, no 3, 185-197 p.Article in journal (Refereed) PublishedText
Abstract [en]

Inhalation anesthesia with isoflurane is a well-established and safe method used in small laboratory animals. In most cases oxygen is used as a carrier gas for isoflurane, but room air or mixtures of oxygen with air or nitrous oxide are also being used. Anesthesia is therefore administered using different fractions of inspired oxygen (FiO(2)), and this may have consequences for the outcome of experiments. The aim of the present study was to investigate the influence of FiO(2) on rat hind limb ischemia/reperfusion injury and to refine the used inhalation anesthesia. Male Wistar rats were subjected to 3.5h of ischemia and 2h of reperfusion, and divided into three groups according to FiO(2) in the O-2/air/isoflurane anesthesia gas mixture: 40%, 60%, and 100% O-2. Normal, healthy rats were used as controls. Muscle edema and creatine kinase MM, a marker for myocyte necrosis, were significantly increased with 40% FiO(2) as compared with 100% FiO(2) (P<0.05). Partial pressure of oxygen, oxygen saturation, and oxyhemoglobin were significantly higher in the 100% O-2 group as compared with 40% O-2. No significant differences were detected for other parameters, such as the oxidative stress markers malondialdehyde and superoxide dismutase. We conclude that a refined inhalation anesthesia setting using 40% FiO(2), reflecting more or less the clinical situation, leads to a more severe and more physiologically relevant reperfusion injury than higher FiO(2.) Oxidative stress did not correlate with FiO(2) and seemed to have no influence on reperfusion injury.

Place, publisher, year, edition, pages
2016. Vol. 50, no 3, 185-197 p.
Keyword [en]
refinement, ischemia, reperfusion injury, fraction of inspired oxygen, rat
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-298073DOI: 10.1177/0023677215604531ISI: 000376294600003PubMedID: 26345513OAI: oai:DiVA.org:uu-298073DiVA: diva2:944845
Available from: 2016-06-30 Created: 2016-06-29 Last updated: 2016-06-30Bibliographically approved

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