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Detection of signal recognition particle (SRP) RNAs in the nuclear ribosomal internal transcribed spacer 1 (ITS1) of three lineages of ectomycorrhizal fungi (Agaricomycetes, Basidiomycota)
Univ Gothenburg, Dept Marine Sci, Bioinformat Infrastruct Life Sci, Box 460, SE-40530 Gothenburg, Sweden..
CSIC, RJB, Dept Micol, Plaza Murillo 1, Madrid 28014, Spain..
Univ Tartu, Inst Ecol & Earth Sci, Ulikooli 18, EE-50090 Tartu, Estonia..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Systematic Biology.
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2016 (English)In: MycoKeys, ISSN 1314-4057, E-ISSN 1314-4049, no 13, 21-33 p.Article in journal (Refereed) PublishedText
Abstract [en]

During a routine scan for Signal Recognition Particle (SRP) RNAs in eukaryotic sequences, we surprisingly found in silico evidence in GenBank for a 265-base long SRP RNA sequence in the ITS1 region of a total of 11 fully identified species in three ectomycorrhizal genera of the Basidiomycota (Fungi): Astraeus, Russula, and Lactarius. To rule out sequence artifacts, one specimen from a species indicated to have the SRP RNA-containing ITS region in each of these genera was ordered and re-sequenced. Sequences identical to the corresponding GenBank entries were recovered, or in the case of a non-original but conspecific specimen differed by three bases, showing that these species indeed have an SRP RNA sequence incorporated into their ITS1 region. Other than the ribosomal genes, this is the first known case of non-coding RNAs in the eukaryotic ITS region, and it may assist in the examination of other types of insertions in fungal genomes.

Place, publisher, year, edition, pages
2016. no 13, 21-33 p.
Keyword [en]
SRP RNA, non-coding RNA, ribosomal RNA, internal transcribed spacer 1 (ITS1), ectomycorrhizal fungi
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-298708DOI: 10.3897/mycokeys.13.8579ISI: 000376791100002OAI: oai:DiVA.org:uu-298708DiVA: diva2:947252
Funder
Swedish Research Council Formas, 215-2011-498Stiftelsen Olle Engkvist Byggmästare
Available from: 2016-07-07 Created: 2016-07-06 Last updated: 2016-07-07Bibliographically approved

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