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Excised segments of rat small intestine in Ussing chamber studies: A comparison of native and stripped tissue viability and permeability to drugs
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
AstraZeneca Innovat Med & Early Dev, DSM Safety & ADME Translat Sci, S-43183 Molndal, Sweden..
AstraZeneca Innovat Med & Early Dev, DSM Safety & ADME Translat Sci, S-43183 Molndal, Sweden..
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2016 (English)In: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 505, no 1-2, 361-368 p.Article in journal (Refereed) PublishedText
Abstract [en]

Excised rat intestinal tissue mounted in an Ussing chamber can be used for intestinal permeability assessments in drug development. The outer layer of the intestine, the serosa and part of the muscle layer, is traditionally removed since it is considered a barrier to the diffusion of nutrients and oxygen as well as to that of pharmaceutical substances. However, the procedure for removing the serosal-muscle layer, i.e. stripping, is a technically challenging process in the pre-experimental preparation of the tissue which may result in tissue damage and reduced viability of the segment. In this study, the viability of stripped and native (non-stripped) rat small intestine tissue segments mounted in Ussing chambers was monitored and the apparent permeability of the tissue to a set of test compounds across both tissue preparations was determined. Electrical measurements, in particular the potential difference (PD) across the intestinal membrane, were used to evaluate the viability. In this study, there were no differences in initial PD (health status of the tissue) or PD over time (viability throughout the experiment) between native and stripped rat jejunum segments. Overall, there were also no significant differences in permeability between stripped and native rat intestinal tissue for the compounds in this study. Based on these results, we propose that stripping can be excluded from the preparation procedures for rat jejunal tissue for permeability studies when using the Ussing chamber technique.

Place, publisher, year, edition, pages
2016. Vol. 505, no 1-2, 361-368 p.
Keyword [en]
Ussing chamber, Rat jejunum, Serosal-muscle layer, Drug permeability
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-298874DOI: 10.1016/j.ijpharm.2016.03.063ISI: 000376695900039PubMedID: 27073083OAI: oai:DiVA.org:uu-298874DiVA: diva2:948296
Available from: 2016-07-11 Created: 2016-07-11 Last updated: 2016-07-11Bibliographically approved

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Sjögren, ErikEriksson, Johanna
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