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Comprehensive Proteomic Study of the Antiproliferative Activity of a Polyphenol-Enriched Rosemary Extract on Colon Cancer Cells Using Nanoliquid Chromatography-Orbitrap MS/MS
CSIC, Inst Food Sci Res CIAL, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
CSIC, Inst Food Sci Res CIAL, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain..
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2016 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 15, no 6, 1971-1985 p.Article in journal (Refereed) PublishedText
Abstract [en]

In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated, in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress..

Place, publisher, year, edition, pages
2016. Vol. 15, no 6, 1971-1985 p.
Keyword [en]
antiproliferative activity, foodomics, colon cancer, dimethyl labeling, HT-29 cells, mass spectrometry, quantitative proteomics, rosemary extract
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-299062DOI: 10.1021/acs.jproteome.6b00154ISI: 000377319200021PubMedID: 27103343OAI: oai:DiVA.org:uu-299062DiVA: diva2:948772
Funder
Swedish Research Council, 2011-4423 2015-4870
Available from: 2016-07-13 Created: 2016-07-13 Last updated: 2016-07-13Bibliographically approved

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Artemenko, Konstantin A.Bergquist, Jonas
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