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Enhancement of poly-3-hydroxybutyrate production in Synechocystis sp PCC 6803 by overexpression of its native biosynthetic genes
Chulalongkorn Univ, Dept Biochem, Lab Cyanobacterial Biotechnol, Fac Sci, Bangkok 10330, Thailand.;Rajamangala Univ Technol Thanyaburi, Fac Sci & Technol, Div Biol, Thanyaburi 12110, Pathumthani, Thailand..
Chulalongkorn Univ, Dept Biochem, Lab Cyanobacterial Biotechnol, Fac Sci, Bangkok 10330, Thailand..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
Chulalongkorn Univ, Dept Biochem, Lab Cyanobacterial Biotechnol, Fac Sci, Bangkok 10330, Thailand..
2016 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 214, 761-768 p.Article in journal (Refereed) PublishedText
Abstract [en]

Synechocystis sp. PCC 6803 strains overexpressing pha genes were constructed and characterized for poly-3- hydroxybutyrate (PHB) production. These pha overexpressing strains showed slightly reduced growth rates. Under N-deprived condition, the strains overexpressing (OE) phaAB, phaEC and phaABEC showed significantly higher PHB contents than the wild type. The maximum PHB content, a 2.6-fold increase producing 26% PHB (dcw), was observed in OE phaAB cells grown for 9 days in N-deprived medium. Under this condition, these OE phaAB cells increased PHB production to 35% PHB (dcw) upon addition of 0.4% (w/v) acetate. Higher PHB granules in OE phaAB cells were clearly visualized by both Nile red staining and TEM imaging. All OE strains under N-deficient condition had increased glgX transcript levels. Overall results demonstrate an enhanced PHB production in Synechocystis cells overexpressing pha genes, particularly phaA and phaB, when grown in N-deprived medium containing 0.4% (w/v) acetate.

Place, publisher, year, edition, pages
2016. Vol. 214, 761-768 p.
Keyword [en]
pha overexpression, Polyhydroxybutyrate, Synechocystis sp PCC 6803
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-299023DOI: 10.1016/j.biortech.2016.05.014ISI: 000377366900096PubMedID: 27213577OAI: oai:DiVA.org:uu-299023DiVA: diva2:948869
Funder
Knut and Alice Wallenberg FoundationSwedish Energy Agency
Available from: 2016-07-14 Created: 2016-07-13 Last updated: 2016-07-14Bibliographically approved

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