uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A gene encoding a potential adenosine 5 '-phosphosulphate kinase is necessary for timely development of Myxococcus xanthus
Southeast Univ, Minist Educ Dev Genes & Human Dis, Key Lab, Dept Biochem,Sch Med, Nanjing 210009, Jiangsu, Peoples R China..
Southeast Univ, Minist Educ Dev Genes & Human Dis, Key Lab, Dept Biochem,Sch Med, Nanjing 210009, Jiangsu, Peoples R China..
Southeast Univ, Minist Educ Dev Genes & Human Dis, Key Lab, Dept Biochem,Sch Med, Nanjing 210009, Jiangsu, Peoples R China..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
Show others and affiliations
2016 (English)In: Microbiology, ISSN 1350-0872, E-ISSN 1465-2080, Vol. 162, 672-683 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

A Myxococcus xanthus gene, MXAN3487, was identified by transposon mutagenesis to be required for the expression of mcuABC, an operon coding for part of the chaperone-usher (CU) system in this bacterium. The MXAN3487 protein displays sequence and structural homology to adenosine 5'-phosphosulphate (APS) kinase family members and contains putative motifs for ATP and APS binding. Although the MXAN3487 locus is not linked to other sulphate assimilation genes, its protein product may have APS kinase activity in vivo and the importance of the ATP-binding site for activity was demonstrated. Expression of MXAN3487 was not affected by sulphate availability, suggesting that MXAN3487 may not function in a reductive sulphate assimilation pathway. Deletion of MXAN3487 significantly delayed fruiting body formation and the production of McuA, a spore coat protein secreted by the M. xanthus Mcu CU system. Based on these observations and data from our previous studies, we propose that MXAN3487 may phosphorylate molecules structurally related to APS, generating metabolites necessary for M. xanthus development, and that MXAN3487 exerts a positive effect on the mcuABC operon whose expression is morphogenesis dependent.

Place, publisher, year, edition, pages
2016. Vol. 162, 672-683 p.
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-299616DOI: 10.1099/mic.0.000254ISI: 000377847000008PubMedID: 26860640OAI: oai:DiVA.org:uu-299616DiVA: diva2:949841
Available from: 2016-07-25 Created: 2016-07-25 Last updated: 2016-07-25Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Knight, Stefan
By organisation
Structure and Molecular Biology
In the same journal
Microbiology
Microbiology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 341 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf