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Epigenomics of Total Acute Sleep Deprivation in Relation to Genome-Wide DNA Methylation Profiles and RNA Expression
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
2016 (English)In: Omics, ISSN 1536-2310, E-ISSN 1557-8100, Vol. 20, no 6, 334-342 p.Article in journal (Refereed) PublishedText
Abstract [en]

Despite an established link between sleep deprivation and epigenetic processes in humans, it remains unclear to what extent sleep deprivation modulates DNA methylation. We performed a within-subject randomized blinded study with 16 healthy subjects to examine the effect of one night of total sleep deprivation (TSD) on the genome-wide methylation profile in blood compared with that in normal sleep. Genome-wide differences in methylation between both conditions were assessed by applying a paired regression model that corrected for monocyte subpopulations. In addition, the correlations between the methylation of genes detected to be modulated by TSD and gene expression were examined in a separate, publicly available cohort of 10 healthy male donors (E-GEOD-49065). Sleep deprivation significantly affected the DNA methylation profile both independently and in dependency of shifts in monocyte composition. Our study detected differential methylation of 269 probes. Notably, one CpG site was located 69 bp upstream of ING5, which has been shown to be differentially expressed after sleep deprivation. Gene set enrichment analysis detected the Notch and Wnt signaling pathways to be enriched among the differentially methylated genes. These results provide evidence that total acute sleep deprivation alters the methylation profile in healthy human subjects. This is, to our knowledge, the first study that systematically investigated the impact of total acute sleep deprivation on genome-wide DNA methylation profiles in blood and related the epigenomic findings to the expression data.

Place, publisher, year, edition, pages
2016. Vol. 20, no 6, 334-342 p.
National Category
Medical Genetics
Identifiers
URN: urn:nbn:se:uu:diva-299600DOI: 10.1089/omi.2016.0041ISI: 000378050700002PubMedID: 27310475OAI: oai:DiVA.org:uu-299600DiVA: diva2:949870
Funder
Swedish Research CouncilThe Swedish Brain FoundationNovo NordiskSwedish Society for Medical Research (SSMF)Stiftelsen Olle Engkvist ByggmästareEU, European Research Council
Available from: 2016-07-25 Created: 2016-07-25 Last updated: 2016-07-25Bibliographically approved

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Nilsson, Emil K.Boström, Adrian E.Mwinyi, JessicaSchiöth, Helgi B.
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