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Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Univ Tsukuba, Fac Med, Dept Expt Pathol, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058577, Japan..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research.
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2016 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 291, no 24, 12706-12723 p.Article in journal (Refereed) PublishedText
Abstract [en]

We previously established a mechanism of negative regulation of transforming growth factor beta signaling mediated by the nuclear ADP-ribosylating enzyme poly-(ADP-ribose) polymerase 1 (PARP1) and the deribosylating enzyme poly-(ADP-ribose) glycohydrolase (PARG), which dynamically regulate ADP-ribosylation of Smad3 and Smad4, two central signaling proteins of the pathway. Here we demonstrate that the bone morphogenetic protein (BMP) pathway can also be regulated by the opposing actions of PARP1 and PARG. PARG positively contributes to BMP signaling and forms physical complexes with Smad5 and Smad4. The positive role PARG plays during BMP signaling can be neutralized by PARP1, as demonstrated by experiments where PARG and PARP1 are simultaneously silenced. In contrast to PARG, ectopic expression of PARP1 suppresses BMP signaling, whereas silencing of endogenous PARP1 enhances signaling and BMP-induced differentiation. The two major Smad proteins of the BMP pathway, Smad1 and Smad5, interact with PARP1 and can be ADP-ribosylated in vitro, whereas PARG causes deribosylation. The overall outcome of this mode of regulation of BMP signal transduction provides a fine-tuning mechanism based on the two major enzymes that control cellular ADP-ribosylation.

Place, publisher, year, edition, pages
2016. Vol. 291, no 24, 12706-12723 p.
National Category
Cell and Molecular Biology
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URN: urn:nbn:se:uu:diva-299724DOI: 10.1074/jbc.M116.729699ISI: 000378119900024PubMedID: 27129221OAI: oai:DiVA.org:uu-299724DiVA: diva2:949999
Funder
Swedish Research Council, K2010-67X-14936-07-3 K2013-66X-14936-10-5
Available from: 2016-07-26 Created: 2016-07-26 Last updated: 2016-07-26Bibliographically approved

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Watanabe, YukihidePapoutsoglou, PanagiotisMaturi, VarunTsubakihara, YutaroHeldin, Carl-HenrikMoustakas, Aristidis
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Department of Pharmaceutical BiosciencesLudwig Institute for Cancer ResearchDepartment of Medical Biochemistry and Microbiology
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