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Surface plasmon resonance biosensing: Approaches for screening and characterising antibodies for food diagnostics
US FDA, Ctr Food Safety & Appl Nutr, 5100 Paint Branch Pkwy, College Pk, MD 20740 USA..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology. GE Healthcare Biosci AB, Bjorkgatan 30, S-75184 Uppsala, Sweden..
Queens Univ Belfast, Sch Biol Sci, Inst Global Food Secur, David Keir Bldg,Stranmillis Rd, Belfast BT9 5AG, Antrim, North Ireland..
Queens Univ Belfast, Sch Biol Sci, Inst Global Food Secur, David Keir Bldg,Stranmillis Rd, Belfast BT9 5AG, Antrim, North Ireland..
2016 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 156, 55-63 p.Article in journal (Refereed) PublishedText
Abstract [en]

Research in biosensing approaches as alternative techniques for food diagnostics for the detection of chemical contaminants and foodborne pathogens has increased over the last twenty years. The key component of such tests is the biorecognition element whereby polyclonal or monoclonal antibodies still dominate the market. Traditionally the screening of sera or cell culture media for the selection of polyclonal or monoclonal candidate antibodies respectively has been performed by enzyme immunoassays. For niche toxin compounds, enzyme immunoassays can be expensive and/or prohibitive methodologies for antibody production due to limitations in toxin supply for conjugate production. Automated, self-regenerating, chip-based biosensors proven in food diagnostics may be utilised as rapid screening tools for antibody candidate selection. This work describes the use of both single channel and multi-channel surface plasmon resonance (SPR) biosensors for the selection and characterisation of antibodies, and their evaluation in shellfish tissue as standard techniques for the detection of domoic acid, as a model toxin compound. The key advantages in the use of these biosensor techniques for screening hybridomas in monoclonal antibody production were the real time observation of molecular interaction and rapid turnaround time in analysis compared to enzyme immunoassays. The multichannel prototype instrument was superior with 96 analyses completed in 2 h compared to 12 h for the single channel and over 24 h for the ELISA immunoassay. Antibodies of high sensitivity, IC50's ranging from 4.8 to 6.9 ng/mL for monoclonal and 2.3-6.0 ng/mL for polyclonal, for the detection of domoic acid in a 1 min analysis time were selected. Although there is a progression for biosensor technology towards low cost, multiplexed portable diagnostics for the food industry, there remains a place for laboratory-based SPR instrumentation for antibody development for food diagnostics as shown herein.

Place, publisher, year, edition, pages
2016. Vol. 156, 55-63 p.
Keyword [en]
Surface plasmon resonance, Antibody, Biosensor, Domoic acid, Marine toxin, Shellfish
National Category
Respiratory Medicine and Allergy
URN: urn:nbn:se:uu:diva-299696DOI: 10.1016/j.talanta.2016.05.008ISI: 000378182900009PubMedID: 27260435OAI: oai:DiVA.org:uu-299696DiVA: diva2:950046
Available from: 2016-07-27 Created: 2016-07-26 Last updated: 2016-07-27Bibliographically approved

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