uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Simultaneous imaging of multiple neurotransmitters and neuroactive substances in the brain by desorption electrospray ionization mass spectrometry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
AstraZeneca, Drug Safety & Metab, Cambridge CB4 0WG, England..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Show others and affiliations
2016 (English)In: NeuroImage, ISSN 1053-8119, E-ISSN 1095-9572, Vol. 136, 129-138 p.Article in journal (Refereed) PublishedText
Abstract [en]

With neurological processes involving multiple neurotransmitters and neuromodulators, it is important to have the ability to directly map and quantify multiple signaling molecules simultaneously in a single analysis. By utilizing a molecular-specific approach, namely desorption electrospray ionization mass spectrometry imaging (DESI-MSI), we demonstrated that the technique can be used to image multiple neurotransmitters and their metabolites (dopamine, dihydroxyphenylacetic acid, 3-methoxytyramine, serotonin, glutamate, glutamine, aspartate,gamma-aminobutyric acid, adenosine) as well as neuroactive drugs (amphetamine, sibutramine, fluvoxamine) and drug metabolites in situ directly in brain tissue sections. The use of both positive and negative ionization modes increased the number of identified molecular targets. Chemical derivatization by charge-tagging the primary amines of molecules significantly increased the sensitivity, enabling the detection of low abundant neurotransmitters and other neuroactive substances previously undetectable by MSI. The sensitivity of the imaging approach of neurochemicals has a great potential in many diverse applications in fields such as neuroscience, pharmacology, drug discovery, neurochemistry, and medicine.

Place, publisher, year, edition, pages
2016. Vol. 136, 129-138 p.
Keyword [en]
Neurotransmitter, Dopamine, Imaging, Mass spectrometry, Parkinson's disease, Serotonin
National Category
Neurology Radiology, Nuclear Medicine and Medical Imaging
Identifiers
URN: urn:nbn:se:uu:diva-299698DOI: 10.1016/j.neuroimage.2016.05.004ISI: 000378048000012PubMedID: 27155126OAI: oai:DiVA.org:uu-299698DiVA: diva2:950048
Funder
Swedish Research Council, 2013-3105 2014-6215 2009-6050The Swedish Brain FoundationSwedish Foundation for Strategic Research , RIF14-0078EU, FP7, Seventh Framework Programme, 607517
Available from: 2016-07-27 Created: 2016-07-26 Last updated: 2016-07-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Shariatgorji, MohammadrezaNilsson, AnnaKallbäck, PatrikVallianatou, TheodosiaAndrén, Per E.
By organisation
Department of Pharmaceutical Biosciences
In the same journal
NeuroImage
NeurologyRadiology, Nuclear Medicine and Medical Imaging

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 44 hits
ReferencesLink to record
Permanent link

Direct link