Simultaneous imaging of multiple neurotransmitters and neuroactive substances in the brain by desorption electrospray ionization mass spectrometry
2016 (English)In: NeuroImage, ISSN 1053-8119, E-ISSN 1095-9572, Vol. 136, 129-138 p.Article in journal (Refereed) PublishedText
With neurological processes involving multiple neurotransmitters and neuromodulators, it is important to have the ability to directly map and quantify multiple signaling molecules simultaneously in a single analysis. By utilizing a molecular-specific approach, namely desorption electrospray ionization mass spectrometry imaging (DESI-MSI), we demonstrated that the technique can be used to image multiple neurotransmitters and their metabolites (dopamine, dihydroxyphenylacetic acid, 3-methoxytyramine, serotonin, glutamate, glutamine, aspartate,gamma-aminobutyric acid, adenosine) as well as neuroactive drugs (amphetamine, sibutramine, fluvoxamine) and drug metabolites in situ directly in brain tissue sections. The use of both positive and negative ionization modes increased the number of identified molecular targets. Chemical derivatization by charge-tagging the primary amines of molecules significantly increased the sensitivity, enabling the detection of low abundant neurotransmitters and other neuroactive substances previously undetectable by MSI. The sensitivity of the imaging approach of neurochemicals has a great potential in many diverse applications in fields such as neuroscience, pharmacology, drug discovery, neurochemistry, and medicine.
Place, publisher, year, edition, pages
2016. Vol. 136, 129-138 p.
Neurotransmitter, Dopamine, Imaging, Mass spectrometry, Parkinson's disease, Serotonin
Neurology Radiology, Nuclear Medicine and Medical Imaging
IdentifiersURN: urn:nbn:se:uu:diva-299698DOI: 10.1016/j.neuroimage.2016.05.004ISI: 000378048000012PubMedID: 27155126OAI: oai:DiVA.org:uu-299698DiVA: diva2:950048
FunderSwedish Research Council, 2013-3105 2014-6215 2009-6050The Swedish Brain FoundationSwedish Foundation for Strategic Research , RIF14-0078EU, FP7, Seventh Framework Programme, 607517