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Visualization and analysis of gene expression in tissue sections by spatial transcriptomics
Karolinska Inst, Dept Cell & Mol Biol, SE-17177 Stockholm, Sweden.;KTH Royal Inst Technol, Div Gene Technol, Sci Life Lab, SE-10691 Stockholm, Sweden..
KTH Royal Inst Technol, Div Gene Technol, Sci Life Lab, SE-10691 Stockholm, Sweden..
KTH Royal Inst Technol, Div Gene Technol, Sci Life Lab, SE-10691 Stockholm, Sweden..
KTH Royal Inst Technol, Div Gene Technol, Sci Life Lab, SE-10691 Stockholm, Sweden.;Karolinska Inst, Div Periodontol, Dept Dent Med, SE-14104 Huddinge, Sweden..
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2016 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 353, no 6294, 78-82 p.Article in journal (Refereed) PublishedText
Abstract [en]

Analysis of the pattern of proteins or messenger RNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call "spatial transcriptomics," that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics.

Place, publisher, year, edition, pages
2016. Vol. 353, no 6294, 78-82 p.
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-299862DOI: 10.1126/science.aaf2403ISI: 000378816200040PubMedID: 27365449OAI: oai:DiVA.org:uu-299862DiVA: diva2:950248
Funder
Knut and Alice Wallenberg FoundationSwedish Foundation for Strategic Research Swedish Research CouncilSwedish Cancer SocietyThe Karolinska Institutet's Research FoundationTorsten Söderbergs stiftelseRagnar Söderbergs stiftelseÅke Wiberg Foundation
Available from: 2016-07-28 Created: 2016-07-28 Last updated: 2016-07-28Bibliographically approved

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Pontén, Fredrik
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Department of Immunology, Genetics and Pathology
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