Clamping of RNA with PNA enables targeting of microRNA
2016 (English)In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 14, no 23, 5210-5213 p.Article in journal (Refereed) PublishedText
To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.
Place, publisher, year, edition, pages
2016. Vol. 14, no 23, 5210-5213 p.
Organic Chemistry Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-299933DOI: 10.1039/c6ob00516kISI: 000378512400003PubMedID: 27203783OAI: oai:DiVA.org:uu-299933DiVA: diva2:950338