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Clamping of RNA with PNA enables targeting of microRNA
Karolinska Inst, Dept Biosci & Nutr, Novum, S-14183 Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Karolinska Inst, Dept Biosci & Nutr, Novum, S-14183 Stockholm, Sweden.;Univ Turku, Dept Chem, Turku 20014, Finland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2016 (English)In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 14, no 23, 5210-5213 p.Article in journal (Refereed) PublishedText
Abstract [en]

To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.

Place, publisher, year, edition, pages
2016. Vol. 14, no 23, 5210-5213 p.
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Organic Chemistry Biochemistry and Molecular Biology
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URN: urn:nbn:se:uu:diva-299933DOI: 10.1039/c6ob00516kISI: 000378512400003PubMedID: 27203783OAI: oai:DiVA.org:uu-299933DiVA: diva2:950338
Available from: 2016-07-29 Created: 2016-07-29 Last updated: 2016-07-29Bibliographically approved

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Punga, Tanel
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