A data set from flash X-ray imaging of carboxysomes
2016 (English)In: Scientific Data, ISSN 1012-0602, E-ISSN 2052-4463, Vol. 3, 160061Article in journal (Refereed) PublishedText
Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.
Place, publisher, year, edition, pages
2016. Vol. 3, 160061
IdentifiersURN: urn:nbn:se:uu:diva-300202DOI: 10.1038/sdata.2016.61OAI: oai:DiVA.org:uu-300202DiVA: diva2:951092
Data Descriptor2016-08-052016-08-052016-09-02Bibliographically approved