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A short phylogenetically informative cpDNA fragment for the identification of Pinus species
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Plant Ecology and Evolution. Aristotle Univ Thessaloniki, Sch Biol, Lab Systemat Bot & Phytogeog, POB 104, GR-54124 Thessaloniki, Greece.;Univ Washington, Sch Med, Div Med Genet, 1795 NE Pacific St,Box 357720, Seattle, WA 98195 USA..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Plant Ecology and Evolution.
Aristotle Univ Thessaloniki, Sch Biol, Lab Systemat Bot & Phytogeog, POB 104, GR-54124 Thessaloniki, Greece..
2016 (English)In: Biochemical Systematics and Ecology, ISSN 0305-1978, E-ISSN 1873-2925, Vol. 66, 166-172 p.Article in journal (Refereed) PublishedText
Abstract [en]

The genus Pinus L. consists of ca. 110 ecologically and economically important species extending from the arctic zone to the tropics. Nevertheless, there is little information in the literature on DNA-based methods for the identification of pine species. Here, we identified a new cpDNA fragment (trnV-H/x-h) able to differentiate among pine species and correctly depict the phylogeny within the genus. The fragment was identified based on PCR-RFLP profiles and primers designed based on the sequences of six Pinus species naturally occurring in Greece (Pinus brutia Ten., Pinus halepensis Mill., Pinus leucodermis Antoine, Pinus nigra J.F. Arnold, Pinus pinea L, and Pinus sylvestris L). We analyzed 90 highly similar pine sequences retrieved from the GenBank to investigate specificity of our marker and the haplotypes found showed to be specific to Pinus and able to differentiate among 39 different species. The phylogenetic tree constructed using these species, correctly depicted the phylogeny of the genus up to the subsection level. These characteristics together with its relatively small size (376-418 bp) make the trnV-H/x-h marker useful for pine identification even in contexts where DNA is degraded, such as in timber tracing, forensic botany and palaeobotanical investigations.

Place, publisher, year, edition, pages
2016. Vol. 66, 166-172 p.
Keyword [en]
Pinus, Species identification, cpDNA marker, trnV-trnH, Phylogeny
National Category
Evolutionary Biology
Identifiers
URN: urn:nbn:se:uu:diva-300564DOI: 10.1016/j.bse.2016.03.001ISI: 000379281300024OAI: oai:DiVA.org:uu-300564DiVA: diva2:951666
Funder
Swedish Research Council, 2007-4490Carl Tryggers foundation , 08:303
Available from: 2016-08-09 Created: 2016-08-09 Last updated: 2016-08-09Bibliographically approved

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