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Structural and functional studies of the glycoside hydrolase family 3 beta-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii
Swedish Univ Agr Sci, Dept Chem & Biotechnol, Box 7015, S-75007 Uppsala, Sweden..
Swedish Univ Agr Sci, Dept Chem & Biotechnol, Box 7015, S-75007 Uppsala, Sweden..
Swedish Univ Agr Sci, Dept Chem & Biotechnol, Box 7015, S-75007 Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
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2016 (English)In: ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, ISSN 2059-7983, Vol. 72, no 7, 860-870 p.Article in journal (Refereed) PublishedText
Abstract [en]

The filamentous fungus Hypocrea jecorina produces a number of cellulases and hemicellulases that act in a concerted fashion on biomass and degrade it into monomeric or oligomeric sugars. beta-Glucosidases are involved in the last step of the degradation of cellulosic biomass and hydrolyse the beta-glycosidic linkage between two adjacent molecules in dimers and oligomers of glucose. In this study, it is shown that substituting the beta-glucosidase from H. jecorina (HjCel3A) with the beta-glucosidase Cel3A from the thermophilic fungus Rasamsonia emersonii (ReCel3A) in enzyme mixtures results in increased efficiency in the saccharification of lignocellulosic materials. Biochemical characterization of ReCel3A, heterologously produced in H. jecorina, reveals a preference for disaccharide substrates over longer gluco-oligosaccharides. Crystallographic studies of ReCel3A revealed a highly N-glycosylated three-domain dimeric protein, as has been observed previously for glycoside hydrolase family 3 beta-glucosidases. The increased thermal stability and saccharification yield and the superior biochemical characteristics of ReCel3A compared with HjCel3A and mixtures containing HjCel3A make ReCel3A an excellent candidate for addition to enzyme mixtures designed to operate at higher temperatures.

Place, publisher, year, edition, pages
2016. Vol. 72, no 7, 860-870 p.
Keyword [en]
glycoside hydrolase, beta-glucosidase, biodegradation, crystal structure, Rasamsonia emersonii, Cel3A, glycoproteins, thermophilic fungus
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-301027DOI: 10.1107/S2059798316008482ISI: 000379912500005PubMedID: 27377383OAI: oai:DiVA.org:uu-301027DiVA: diva2:953300
Available from: 2016-08-17 Created: 2016-08-17 Last updated: 2016-08-17Bibliographically approved

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