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Bacterial diversity across a highly stratified ecosystem: A salt-wedge Mediterranean estuary
Rudjer Boskovic Inst, Ctr Marine Res, Rovinj, Croatia.
Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Palaeobiology.
Rudjer Boskovic Inst, Div Marine & Environm Res, Zagreb, Croatia.
Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Palaeobiology.
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2016 (English)In: Systematic and Applied Microbiology, ISSN 0723-2020, E-ISSN 1618-0984, Vol. 39, no 6, 398-408 p.Article in journal (Refereed) Published
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Abstract [en]

Highly stratified Mediterranean estuaries are unique environments where the tidal range is low and the tidal currents are almost negligible. The main characteristics of these environments are strong salinity gradients and other environmental parameters. In this study, 454 pyrosequencing of the 16S rRNA gene in combination with catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) was used to estimate the bacterial diversity across the Krka estuary in February and July 2013. The comparison of the data derived from these two techniques resulted in a significant but weak positive correlation (R = 0.28) indicating a substantial difference in the bacterial community structure, depending on the applied method. The phytoplankton bloom observed in February was identified as one of the main factors shaping the bacterial community structure between the two environmentally contrasting sampling months. Roseobacter, Bacteroidetes and Gammaproteobacteria differed substantially between February and July. Typical freshwater bacterial classes (Actinobacteria and Betaproteobacteria) showed strong vertical distribution patterns depending on the salinity gradient. Cyanobacteria decreased in abundance in February due to competition with phytoplankton, while the SAR11 clade increased its abundance in July as a result of a better adaptation toward more oligotrophic conditions. The results provided the first detailed insight into the bacterial diversity in a highly stratified Mediterranean karstic estuary.

Place, publisher, year, edition, pages
2016. Vol. 39, no 6, 398-408 p.
Keyword [en]
16S amplicon, CARD FISH, Estuaries, Gradient, Phytoplankton bloom, Diversity
National Category
Microbiology Ecology
Research subject
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-301115DOI: 10.1016/j.syapm.2016.06.006ISI: 000383305000006PubMedID: 27475818OAI: oai:DiVA.org:uu-301115DiVA: diva2:953642
Funder
Knut and Alice Wallenberg Foundation, 2009.0287
Available from: 2016-08-18 Created: 2016-08-18 Last updated: 2016-10-20Bibliographically approved

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