Synthesis of tethered-polymer brush by atom transfer radical polymerization from a plasma-polymerized-film-coated quartz crystal microbalance and its application for immunosensors
2004 (English)In: Biosensors and Bioelectronics, no 20, 1165-1176 p.Article in journal (Refereed) Published
This study synthesizes a tethered surface-grafted poly(acrylic acid) with quartz crystal microbalance (QCM) surfaces and provides detailed analysis of their properties and application. A tethered polyelectrolyte brush of poly (acrylic acid) is generated by first covering the substrate with a plasma-polymerized allyl alcohol (pp-AA) film, changing the polymerization initiators
(bromination) and then grafting through atom transfer radical polymerization (ATRP) of tert-butyl acrylate (t-BA); these initiators are immobilized on a surface and exposed to a monomer. Finally, we convert the poly(t-BA) brush into poly(acrylic acid) through hydrolysis. We use the QCM technique to measure configuration change of the tethered poly(acrylic acid) grafted chains with two differerent degrees of polymerzation (DP 50, 200) in aqueous solutions at three different pH values (4.0, 4.8, and 5.4). The tethered poly(acrylic acid) grafted QCM shows that repeatable frequency responsesare induced by pH change of solution. These frequency responses of large DP for pH are 20 times larger than responses of lower DP for pH.
The frequency response of antibody immobilization on tethered poly(acrylic acid) grafted QCM (DP = 200) and its frequency responses are immunoreaction are 10 times larger than conventional immobilization methods by cysteamine with glutalaldehyde coupling of the antibody. The tethered poly(acrylic acid) grafted QCM can increase the frequency response for pH, the immobilization amount of antibody, and immunosensor response.
Place, publisher, year, edition, pages
2004. no 20, 1165-1176 p.
Plasma-polymerized allyl alcohol, ATRP, Quartz crystal microbalance, Tethered poly(acrylic acid), pH response
IdentifiersURN: urn:nbn:se:uu:diva-67574OAI: oai:DiVA.org:uu-67574DiVA: diva2:95485