uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Intracellular distribution of an integral nuclear pore membrane protein fused to green fluorescent protein--localization of a targeting domain
Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Centre for Image Analysis. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computerized Image Analysis.
Show others and affiliations
1997 (English)In: European Journal of Biochemistry, ISSN 0014-2956, Vol. 250, no 3, 808-13 p.Article in journal (Refereed) Published
Abstract [en]

The 121-kDa pore membrane protein (POM121) is a bitopic integral membrane protein specifically located in the pore membrane domain of the nuclear envelope with its short N-terminal tail exposed on the luminal side and its major C-terminal portion adjoining the nuclear pore complex. In order to locate a signal for targeting of POM121 to the nuclear pores, we overexpressed selected regions of POM121 alone or fused to the green fluorescent protein (GFP) in transiently transfected COS-1 cells or in a stably transfected neuroblastoma cell line. Microscopic analysis of the GFP fluorescence or immunostaining was used to determine the intracellular distribution of the overexpressed proteins. The endofluorescent GFP tag had no effect on the distribution of POM121, since the chimerical POM121-GFP fusion protein was correctly targeted to the nuclear pores of both COS-1 cells and neuroblastoma cells. Based on the differentiated intracellular sorting of the POM121 variants, we conclude that the first 128 amino acids of POM121 contains signals for targeting to the continuous endoplasmic reticulum/nuclear envelope membrane system but not specifically to the nuclear pores and that a specific nuclear pore targeting signal is located between amino acids 129 and 618 in the endoplasmically exposed portion of POM121.

Place, publisher, year, edition, pages
1997. Vol. 250, no 3, 808-13 p.
Keyword [en]
Animals, Blotting; Western, CHO Cells, Green Fluorescent Proteins, Hamsters, Immunohistochemistry, Luminescent Proteins/*analysis/genetics, Membrane Proteins/*analysis/chemistry/genetics, Microscopy; Fluorescence, Nuclear Proteins/analysis, Peptide Fragments/analysis, Recombinant Fusion Proteins/analysis/genetics, Research Support; Non-U.S. Gov't, Transfection/genetics, Tumor Cells; Cultured
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-67594PubMedID: 9461306OAI: oai:DiVA.org:uu-67594DiVA: diva2:95505
Available from: 2008-01-07 Created: 2008-01-07 Last updated: 2010-06-22Bibliographically approved

Open Access in DiVA

No full text

Other links

PubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=9461306&dopt=Citation

Authority records BETA

Wählby (nee Linnman), Carolina

Search in DiVA

By author/editor
Wählby (nee Linnman), Carolina
By organisation
Centre for Image AnalysisComputerized Image Analysis
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 293 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf