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Single skeletal muscle fiber behavior after a quick stretch in young and older men: a possible explanation of the relative preservation of eccentric force in old age
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Clinical Neurophysiology. Muscle Cell Physiology Laboratory, Department of Physical Medicine and RehabilitationSpaulding Rehabilitation Hospital and Harvard Medical School.; Equipe INSERM- ERM 207 Motricité-Plasticité, Faculté des Sciences du SportUniversité de Bourgogne .
Massachusetts Gen Hosp, Ctr Biostat, Boston, MA 02114 USA.
Muscle Cell Physiology Laboratory, Department of Physical Medicine and RehabilitationSpaulding Rehabilitation Hospital and Harvard Medical School.
Muscle Cell Physiology Laboratory, Department of Physical Medicine and RehabilitationSpaulding Rehabilitation Hospital and Harvard Medical School.
2006 (English)In: Pflügers Archiv: European Journal of Physiology, ISSN 0031-6768, E-ISSN 1432-2013, Vol. 452, no 4, 464-470 p.Article in journal (Refereed) Published
Abstract [en]

The origins of the smaller age-related decrease in eccentric force compared to isometric and concentric conditions in vivo remain unclear. Could this originate from contractile elements of muscle cells? The main intent of the current investigation was to assess the force behavior of muscle cells with aging, during lengthening. Chemically skinned single muscle fibers (n=235) from m. vastus lateralis of six young (mean age 31.6 years) and six older men (mean age 66.1 years) were maximally activated with pCa 4.5 at 15 degrees C. Maximal isometric force and cross-sectional area were measured allowing the calculation of the tension (T-0). A quick stretch (2 nm per half-sarcomere length) was applied and caused an immediate increase in tension followed by a decrease and a secondary delayed and transient rise in tension (phase 3); finally, the tension recovered a steady state value (phase 4). The tension enhancements during phase 3 (Delta T (3)) and phase 4 (Delta T (4)) were evaluated. The myosin heavy-chain isoform composition of each single fiber was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Delta T (3) and Delta T (4) were preserved in older men for both type I and IIa fibers despite a reduction in T-0. Therefore, the age-related preservation of the tension increments after a quick stretch in single muscle fibers could explain in part the smaller decrease in force during eccentric contractions compared to isometric and concentric conditions in vivo with aging usually observed.

Place, publisher, year, edition, pages
2006. Vol. 452, no 4, 464-470 p.
Keyword [en]
aging, skinned single muscle fiber, mechanics, stretch response
National Category
Physiology
Identifiers
URN: urn:nbn:se:uu:diva-301872DOI: 10.1007/s00424-006-0065-6ISI: 000238522600011PubMedID: 16622703OAI: oai:DiVA.org:uu-301872DiVA: diva2:955495
Available from: 2016-08-25 Created: 2016-08-25 Last updated: 2016-08-25Bibliographically approved

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