Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins
2004 (English)In: Journal of General Virology, ISSN 0022-1317, E-ISSN 1465-2099, Vol. 85, no Pt 1, 185-196 p.Article in journal (Refereed) Published
We have examined the subcellular localization properties of human adenovirus 2 (HAdV-2) preMu and mature Mu (pX) proteins as fusions with enhanced green fluorescence protein (EGFP). We determined that preMu is exclusively a nucleolar protein with a single nucleolar accumulation signal within the Mu sequence. In addition, we noted that both preMu-EGFP and Mu-EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu-EGFP (but not Mu-EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes. Further investigation suggested this might be due to a failure to express pre-terminal protein (preTP) from the E2 region, despite expression of another E2 protein, DBP. Deletion mutagenesis identified a highly conserved region in the C terminus of preMu responsible for these observations. Thus our data suggest that preMu may play a role in modulating accumulation of proteins from the E2 region.
Place, publisher, year, edition, pages
2004. Vol. 85, no Pt 1, 185-196 p.
Adenovirus E2 Proteins/*metabolism, Adenoviruses; Human/pathogenicity, Amino Acid Sequence, Cell Nucleolus/*metabolism, DNA-Binding Proteins/*metabolism, Gene Deletion, Gene Expression Regulation; Viral, Green Fluorescent Proteins, Hela Cells, Humans, Luminescent Proteins/genetics/metabolism, Molecular Sequence Data, Peptides/*metabolism, Protein Precursors/*metabolism, Recombinant Fusion Proteins/metabolism, Research Support; Non-U.S. Gov't, Viral Core Proteins/*metabolism
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-68010DOI: 10.1099/vir.0.19352-0PubMedID: 14718634OAI: oai:DiVA.org:uu-68010DiVA: diva2:95921