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Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2004 (English)In: Journal of General Virology, ISSN 0022-1317, E-ISSN 1465-2099, Vol. 85, no Pt 1, 185-196 p.Article in journal (Refereed) Published
Abstract [en]

We have examined the subcellular localization properties of human adenovirus 2 (HAdV-2) preMu and mature Mu (pX) proteins as fusions with enhanced green fluorescence protein (EGFP). We determined that preMu is exclusively a nucleolar protein with a single nucleolar accumulation signal within the Mu sequence. In addition, we noted that both preMu-EGFP and Mu-EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu-EGFP (but not Mu-EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes. Further investigation suggested this might be due to a failure to express pre-terminal protein (preTP) from the E2 region, despite expression of another E2 protein, DBP. Deletion mutagenesis identified a highly conserved region in the C terminus of preMu responsible for these observations. Thus our data suggest that preMu may play a role in modulating accumulation of proteins from the E2 region.

Place, publisher, year, edition, pages
2004. Vol. 85, no Pt 1, 185-196 p.
Keyword [en]
Adenovirus E2 Proteins/*metabolism, Adenoviruses; Human/pathogenicity, Amino Acid Sequence, Cell Nucleolus/*metabolism, DNA-Binding Proteins/*metabolism, Gene Deletion, Gene Expression Regulation; Viral, Green Fluorescent Proteins, Hela Cells, Humans, Luminescent Proteins/genetics/metabolism, Molecular Sequence Data, Peptides/*metabolism, Protein Precursors/*metabolism, Recombinant Fusion Proteins/metabolism, Research Support; Non-U.S. Gov't, Viral Core Proteins/*metabolism
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-68010DOI: 10.1099/vir.0.19352-0PubMedID: 14718634OAI: oai:DiVA.org:uu-68010DiVA: diva2:95921
Available from: 2005-02-09 Created: 2005-02-09 Last updated: 2011-12-01Bibliographically approved

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Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=14718634&dopt=Citation

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Akusjärvi, Göran
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