uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Genetic Analysis of Physcomitrella patens Identifies ABSCISIC ACID NON-RESPONSIVE, a Regulator of ABA Responses Unique to Basal Land Plants and Required for Desiccation Tolerance
Univ Leeds, Fac Biol Sci, Ctr Plant Sci, Leeds LS2 9JT, W Yorkshire, England..
Univ Leeds, Fac Biol Sci, Ctr Plant Sci, Leeds LS2 9JT, W Yorkshire, England..
Univ Leeds, Astbury Ctr Struct Mol Biol, Fac Biol Sci, Leeds LS2 9JT, W Yorkshire, England..
Joint Genome Inst, Dept Energy, Walnut Creek, CA 94598 USA.;HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA..
Show others and affiliations
2016 (English)In: The Plant Cell, ISSN 1040-4651, E-ISSN 1532-298X, Vol. 28, no 6, 1310-1327 p.Article in journal (Refereed) Published
Abstract [en]

The anatomically simple plants that first colonized land must have acquired molecular and biochemical adaptations to drought stress. Abscisic acid (ABA) coordinates responses leading to desiccation tolerance in all land plants. We identified ABA nonresponsive mutants in the model bryophyte Physcomitrella patens and genotyped a segregating population to map and identify the ABA NON-RESPONSIVE (ANR) gene encoding a modular protein kinase comprising an N-terminal PAS domain, a central EDR domain, and a C-terminal MAPKKK-like domain. anr mutants fail to accumulate dehydration tolerance-associated gene products in response to drought, ABA, or osmotic stress and do not acquire ABA-dependent desiccation tolerance. The crystal structure of the PAS domain, determined to 1.7-angstrom resolution, shows a conserved PAS-fold that dimerizes through a weak dimerization interface. Targeted mutagenesis of a conserved tryptophan residue within the PAS domain generates plants with ABA nonresponsive growth and strongly attenuated ABA-responsive gene expression, whereas deleting this domain retains a fully ABA-responsive phenotype. ANR orthologs are found in early-diverging land plant lineages and aquatic algae but are absent from more recently diverged vascular plants. We propose that ANR genes represent an ancestral adaptation that enabled drought stress survival of the first terrestrial colonizers but were lost during land plant evolution.

Place, publisher, year, edition, pages
2016. Vol. 28, no 6, 1310-1327 p.
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-302714DOI: 10.1105/tpc.16.00091ISI: 000380689400011PubMedID: 27194706OAI: oai:DiVA.org:uu-302714DiVA: diva2:966158
Available from: 2016-09-08 Created: 2016-09-08 Last updated: 2016-09-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Larsson, Anders
By organisation
Systematic Biology
In the same journal
The Plant Cell
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 1 hits
ReferencesLink to record
Permanent link

Direct link