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Haptophyte diversity and vertical distribution explored by 18S and 28S ribosomal RNA gene metabarcoding and scanning electron microscopy
Univ Oslo, Dept Biosci, POB 1066 Blindern, N-0316 Oslo, Norway.
Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Palaeobiology.ORCID iD: 0000-0003-2613-945X
Univ Oslo, Dept Biosci, POB 1066 Blindern, N-0316 Oslo, Norway.
Univ Oslo, Dept Biosci, POB 1066 Blindern, N-0316 Oslo, Norway.
2017 (English)In: Journal of Eukaryotic Microbiology, ISSN 1066-5234, E-ISSN 1550-7408, Vol. 64, no 4, p. 514-532Article in journal (Refereed) Published
Abstract [en]

Haptophyta encompasses more than 300 species of mostly marine pico- and nanoplanktonic flagellates. Our aims were to investigate the Oslofjorden haptophyte diversity and vertical distribution by metabarcoding, and to improve the approach to study haptophyte community composition, richness and proportional abundance by comparing two rRNA markers and scanning electron microscopy (SEM). Samples were collected in August 2013 at the Outer Oslofjorden, Norway. Total RNA/cDNA was amplified by haptophyte-specific primers targeting the V4 region of the 18S, and the D1-D2 region of the 28S rRNA. Taxonomy was assigned using curated haptophyte reference databases and phylogenetic analyses. Both marker genes showed Chrysochromulinaceae and Prymnesiaceae to be the families with highest number of Operational Taxonomic Units (OTUs), as well as proportional abundance. The 18S rRNA data setalso contained OTUs assigned to eight supported and defined clades consisting of environmental sequences only, possibly representing novel lineages from family to class. We also recorded new species for the area. Comparing coccolithophores by SEM with metabarcoding shows a good correspondence with the 18S rRNA gene proportional abundances. Our results contribute to link morphological and molecular data and 28S to 18S rRNA gene sequences of haptophytes without cultured representatives, and to improve metabarcoding methodology.

Place, publisher, year, edition, pages
2017. Vol. 64, no 4, p. 514-532
Keyword [en]
Abundance, coccolithophores, high-throughput sequencing, Oslofjorden, phylogeny, richness
National Category
Biological Sciences Biological Systematics Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-302898DOI: 10.1111/jeu.12388ISI: 000406102000010PubMedID: 27973742OAI: oai:DiVA.org:uu-302898DiVA: diva2:968787
Note

De två första författarna delar förstaförfattarskapet.

Available from: 2016-09-12 Created: 2016-09-12 Last updated: 2017-11-02Bibliographically approved
In thesis
1. Phenotypic evolution and adaptive strategies in marine phytoplankton (Coccolithophores)
Open this publication in new window or tab >>Phenotypic evolution and adaptive strategies in marine phytoplankton (Coccolithophores)
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Coccolithophores are biogeochemically important marine algae that interact with the carbon cycle through photosynthesis (CO2 sink), calcification (CO2 source) and burial of carbon into oceanic sediments. The group is considered susceptible to the ongoing climate perturbations, in particular to ocean acidification, temperature increase and nutrient limitation. The aim of this thesis was to investigate the adaptation of coccolithophores to environmental change, with the focus on temperature stress and nutrient limitation. The research was conducted in frame of three approaches: experiments testing the physiological response of coccolithophore species Helicosphaera carteri and Coccolithus pelagicus to phosphorus limitation, field studies on coccolithophore life-cycles with a method comparison and an investigation of the phenotypic evolution of the coccolithophore genus Helicosphaera over the past 15 Ma. Experimental results show that the physiology and morphology of large coccolithophores are sensitive to phosphorus limitation, and that the adaptation to low-nutrient conditions can lead to a decrease in calcification rates. Field studies have contributed to our understanding of coccolithophore life cycles, revealing complex ecological patterns within the Mediterranean community which are seemingly regulated by seasonal, temperature-driven environment changes. In addition, the high-throughput sequencing (HTS) molecular method was shown to provide overall good representation of coccolithophore community composition. Finally, the study on Helicosphaera evolution showed that adaptation to decreasing CO2 in higher latitudes involved cell and coccolith size decrease, whereas the adaptation in tropical ecosystems also included a physiological decrease in calcification rates in response to nutrient limitation. This thesis advanced our understanding of coccolithophore adaptive strategies and will improve our predictions on the fate of the group under ongoing climate change.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2016. p. 54
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1427
Keyword
Coccolithophores, Life-Cycle, Phytoplankton, Nutrient limitation, Temperature, Microscopy, High-throughput sequencing, Taxonomy
National Category
Evolutionary Biology Environmental Sciences
Identifiers
urn:nbn:se:uu:diva-302903 (URN)978-91-554-9689-0 (ISBN)
Public defence
2016-10-28, Hambergsalen, Department of Earth Sciences, Villavägen 16, Uppsala, 13:00 (English)
Opponent
Supervisors
Available from: 2016-10-07 Created: 2016-09-12 Last updated: 2016-10-11

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