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Site-specific labelling of anti-HER2 ADAPT proteins for molecular imaging applications
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2016 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Cancer is one of the leading causes of death worldwide. It is of great importance to use reliable diagnostic methods before determining treatment methods. Molecular imaging is a non-invasive approach of visualising tumours in vivo. This method requires imaging agents that are detectable and binds specifically to the tumour. The aim of this degree project was to produce and characterise a protein-based imaging agent, called ADAPT, that has been engineered to bind the breast cancer associated receptor HER2. This protein has previously been labelled with radionuclides in the N-terminal and tested in vivo with promising results. However, due to the fact that the position of the radiolabel can affect the biodistribution of the imaging agent, a comparison to C-terminal radiolabelled variants is of high interest. Radiolabelling can be performed by first attaching a chelator to the protein and later bind the radionuclide through the chelator. The chelator used in this project binds to a thiol group and therefore a unique cysteine was added to the C-termini. The N-termini were altered to both generate variants with and without purification tag. Proteins with altered N-termini and C-termini were produced and later characterised by circular dichroism and surface plasmon resonance. None of the non-tagged variants could be produced to a sufficient amount and was discarded for further studies. However, all variants with a purification tag could be produced and the shortest variant was chosen to be tested in vivo.

Place, publisher, year, edition, pages
UPTEC X, 16 027
Keyword [en]
cancer diagnostics, molecular imaging, ADAPT, HER2
National Category
Natural Sciences Engineering and Technology
URN: urn:nbn:se:uu:diva-302905OAI: oai:DiVA.org:uu-302905DiVA: diva2:968814
External cooperation
Kungliga Tekniska högskolan
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2016-09-15 Created: 2016-09-12 Last updated: 2016-09-15Bibliographically approved

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