uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique
Univ Eduardo Mondlane, Fac Med, Dept Microbiol, Maputo, Mozambique.;Karolinska Inst, Microbiol Tumour & Cell Biol, Stockholm, Sweden..
Univ Eduardo Mondlane, Fac Med, Dept Microbiol, Maputo, Mozambique.;Univ Calif San Diego, Dept Med, San Diego, CA 92103 USA.;Univ Lurio, Fac Ciencias Saude, Nampula, Mozambique..
Univ Eduardo Mondlane, Fac Vet, Dept Paraclin, Maputo, Mozambique..
Karolinska Inst, Microbiol Tumour & Cell Biol, Stockholm, Sweden.;Publ Hlth Agcy Sweden, Solna, Sweden..
Show others and affiliations
2016 (English)In: Journal of Helminthology, ISSN 0022-149X, E-ISSN 1475-2697, Vol. 90, no 5, 630-633 p.Article in journal (Refereed) Published
Abstract [en]

The diagnosis of neurocysticercosis (NCC) requires expensive neuroimaging techniques that are seldom affordable for people in endemic countries. Accordingly, there is a need for new low-cost diagnostic methods that offer high sensitivity and specificity. In this study, we evaluated Western blot analysis of the previously described recombinant antigen Tsol-p27 in relation to a commercial or in-house enzyme-linked immunosorbent assay (ELISA) for NCC, and compared the results with those provided by a commercial enzyme-linked immunoelectrotransfer blot (EITB) assay, which was regarded as the reference standard method. The analysed serum samples were obtained from 165 people, 18 of whom were confirmed to be NCC positive by EITB. Comparing our Western blot analysis of Tsol-p27 with a previous evaluation performed in Central America showed similar specificity (96.69% versus 97.8%) and sensitivity (85.71% versus 86.7%). The present results indicate that the recombinant Tsol-p27 antigen provides good sensitivity and specificity, and might be preferable as a diagnostic antigen in poorly equipped laboratories in endemic countries.

Place, publisher, year, edition, pages
2016. Vol. 90, no 5, 630-633 p.
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:uu:diva-303032DOI: 10.1017/S0022149X15000747ISI: 000381270700016PubMedID: 26292608OAI: oai:DiVA.org:uu-303032DiVA: diva2:970758
Funder
Sida - Swedish International Development Cooperation Agency, 51140011
Available from: 2016-09-14 Created: 2016-09-14 Last updated: 2016-09-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Cell and Molecular Biology
In the same journal
Journal of Helminthology
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 21 hits
ReferencesLink to record
Permanent link

Direct link