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High Concentrations of the Angiogenic Peptide VEGF-A in Seminal Fluid and its Association to Prostasomes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
Sophia Hosp, Androl Lab, Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
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2016 (English)In: Clinical Laboratory, ISSN 1433-6510, Vol. 62, no 8, 1515-1520 p.Article in journal (Refereed) Published
Abstract [en]

Background: Angiogenesis is the formation of new blood vessels by capillary sprouting from pre-existing vessels. This process is associated with increased expression of angiogenic factors like vascular endothelial growth factor (VEGF). The VEGF family consists of five members denoted VEGF-A, B, C, D and placenta growth factor (PlGF). Prostasomes are exosome-like extracellular vesicles existing in seminal plasma. The present study aimed at investigating the possible relationship between VEGF-A in seminal fluid and blood plasma and the prostasomal association of VEGF-A.

Methods: Measurement of VEGF-A concentrations was carried out in seminal plasma from 40 males and in blood plasma from 40 male blood donors utilizing commercial ELISA kits. The prostasomal association of VEGF-A was investigated by flow cytometry.

Results: We found highly elevated concentrations of VEGF-A in seminal fluid (median value 150000 pg/mL) compared with those of blood plasma. Flow cytometric analysis showed that VEGF-A is bound to the surface of prostasomes.

Conclusions: Prostasomes and seminal plasma contain the angiogenic factor VEGF-A in high concentrations exceeding that of blood plasma by 1000 times.

Place, publisher, year, edition, pages
2016. Vol. 62, no 8, 1515-1520 p.
Keyword [en]
angiogenesis factor, ELISA, prostasomes, seminal fluid, vascular endothelial growth factor
National Category
Urology and Nephrology
Identifiers
URN: urn:nbn:se:uu:diva-303148DOI: 10.7754/Clin.Lab.2016.151229ISI: 000381716800017PubMedID: 28164613OAI: oai:DiVA.org:uu-303148DiVA: diva2:970940
Available from: 2016-09-15 Created: 2016-09-15 Last updated: 2017-03-31Bibliographically approved

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