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Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Regulates Ca2+-Influx and Insulin Secretion from Pancreatic beta Cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
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2016 (English)In: CELL CHEMICAL BIOLOGY, ISSN 2451-9448, Vol. 23, no 7, 816-826 p.Article in journal (Refereed) Published
Abstract [en]

Insulin secretion from pancreatic beta cells is regulated by the blood glucose concentration and occurs through Ca2+-triggered exocytosis. The activities of multiple ion channels in the beta cell plasma membrane are required to fine-tune insulin secretion in order to maintain normoglycemia. Phosphoinositide lipids in the plasma membrane often gate ion channels, and variations in the concentration of these lipids affect ion-channel open probability and conductance. Using light-regulated synthesis or depletion of plasma membrane phosphatidylinositol 4,5-bisphosphate (PI[4,5]P-2), we found that this lipid positively regulated both depolarization- and glucose-triggered Ca2+ influx in a dose-dependent manner. Small reductions of PI(4,5)P-2 caused by brief illumination resulted in partial suppression of Ca2+ influx that followed the kinetics of the lipid, whereas depletion resulted in marked inhibition of both Ca2+ influx and insulin secretion.

Place, publisher, year, edition, pages
2016. Vol. 23, no 7, 816-826 p.
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-303122DOI: 10.1016/j.chembiol.2016.06.009ISI: 000381509200010PubMedID: 27447049OAI: oai:DiVA.org:uu-303122DiVA: diva2:971247
Funder
Swedish Research CouncilGöran Gustafsson Foundation for Research in Natural Sciences and MedicineSwedish Diabetes AssociationNovo NordiskEXODIAB - Excellence of Diabetes Research in Sweden
Note

De två första författarna delar förstaförfattarskapet.

Available from: 2016-09-15 Created: 2016-09-15 Last updated: 2016-09-15Bibliographically approved

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Xie, BeichenNguyen, Phuoc MyGucek, AlenkaThonig, AntjeBarg, SebastianIdevall-Hagren, Olof
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