uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Changes in the Photosystem II complex associated with hydrogen formation in sulfur deprived Chlamydomonas reinhardtii
Lomonosov Moscow State Univ, Fac Biol, Dept Biophys, Moscow 119991, Russia..
Univ Bielefeld, Dept Biol, Ctr Bitoechnol CeBiTec, D-33501 Bielefeld, Germany..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
2016 (English)In: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, ISSN 2211-9264, Vol. 18, 296-304 p.Article in journal (Refereed) Published
Abstract [en]

Redox properties of the acceptor side of Photosystem II were studied during H-2 gas production in cells of Chlamydomonas reinhardtii. Flash-induced variable fluorescence changes and thermoluminescence measurements were performed in wild type and Stm6 mutant cells during different stages of sulfur (S)-deprivation. Analysis of the fluorescence decay kinetics indicated that the forward electron transfer on the acceptor side of Photosystem II was dramatically slowed down during the O-2 evolution and O-2 consumption stages and was completely blocked in the anaerobic stage of S-deprivation, thus, indicating a complete reduction of the PQ-pool. During the H-2 formation stage, the forward electron transfer kinetics in the mu sec and msec time scale re-appeared indicating partially restored electron flow from Q(A)(-) to Q(B) and the PQ-pool. Thermoluminescese measurements fully confirmed the fluorescence kinetic analysis. Activation of hydrogenase in the H-2 formation stage is responsible for re-oxidation of the PQ pool and reactivation of the electron flow which was found to be faster and more efficient on the Stm6 mutant due to the higher amount of functionally preserved Photosystem II.

Place, publisher, year, edition, pages
2016. Vol. 18, 296-304 p.
Keyword [en]
Photosystem II, Chlamydomonas reinhardtii, Sulfur deprivation, H-2 production, Fluorescence, Thermoluminescence
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:uu:diva-303256DOI: 10.1016/j.algal.2016.06.025ISI: 000381748800035OAI: oai:DiVA.org:uu-303256DiVA: diva2:971499
Funder
Swedish Energy Agency, 11674-5Knut and Alice Wallenberg Foundation, KAW 2011.0067Carl Tryggers foundation , CTS 14:314
Available from: 2016-09-16 Created: 2016-09-15 Last updated: 2016-09-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Styring, StenbjörnMamedov, Fikret
By organisation
Molecular Biomimetics
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 15 hits
ReferencesLink to record
Permanent link

Direct link