A novel turbidimetric immunoassay for fecal calprotectin optimized for routine chemistry analyzers
2016 (English)In: Journal of clinical laboratory analysis (Print), ISSN 0887-8013, E-ISSN 1098-2825Article in journal (Refereed) Epub ahead of print
BACKGROUND: Fecal calprotectin assays are widely used to exclude inflammatory bowel disease (IBD) in patients with suspected IBD. A problem with the fecal calprotectin assays is the rather long test-turnaround times. A particle enhanced turbidimetric immunoassays (PETIA) for fecal calprotectin would reduce test-turnaround times and would permit more laboratories to perform the measurements. The aim of this study was to evaluate a new feces calprotectin PETIA.
METHOD: Using routine fecal samples the feces calprotectin PETIA was validated on two chemistry analyzers, Mindray BS-380 and Cobas 501.
RESULTS: The assay is linear in the range 11-2000 μg/g, with a limit of quantitation of approximately 10 μg/g. No antigen excess hook effect was observed up to 10 000-15 000 μg/g depending on the instrument used. The turbidimetric method showed a good agreement with the Bühlmann ELISA. The total coefficient of variation was 3%-8% in the 50-100 μg/g range.
CONCLUSION: The fecal calprotectin PETIA, fCal Turbo, is well suited for rapid analysis of fecal calprotectin on Mindray BS-380 or Cobas 501 clinical chemistry analyzers. The test results are commutable with Bühlmann fecal MRP8/14 ELISA.
Place, publisher, year, edition, pages
Clinical Laboratory Medicine
IdentifiersURN: urn:nbn:se:uu:diva-303897DOI: 10.1002/jcla.22061PubMedID: 27629827OAI: oai:DiVA.org:uu-303897DiVA: diva2:974522