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Protective DNA vaccination against experimental autoimmune encephalomyelitis is associated with induction of IFNbeta
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Lobell / Kämpe)
2004 (English)In: Journal of Neuroimmunology, ISSN 0165-5728, E-ISSN 1872-8421, Vol. 149, no 1-2, 66-76 p.Article in journal (Other academic) Published
Abstract [en]

DNA vaccines encoding encephalitogenic peptides protect against subsequent development of rat experimental autoimmune encephalomyelitis (EAE) through unknown mechanisms. We investigated immune cell phenotypes at different time points after DNA vaccination with vaccine encoding myelin oligodendrocyte glycoprotein peptide 91-108 and subsequent induction of EAE. In protected rats, we observed (i) no alterations in antigen-specific Th2 or Th3 responses, (ii) reduced MHC II expression on splenocytes early after EAE induction, (iii) antigen-specific upregulation of IFNbeta upon recall stimulation and (iv) reduced IL-12Rbeta2 on lymphocytes. We suggest that the underlying mechanism of DNA vaccination is associated with immunomodulation exerted by induced IFNbeta.

Place, publisher, year, edition, pages
2004. Vol. 149, no 1-2, 66-76 p.
Keyword [en]
Animals, Antigens; Surface/genetics/metabolism, Cells; Cultured, Central Nervous System/cytology, Comparative Study, Cytokines/genetics/metabolism, Disease Models; Animal, Encephalomyelitis; Autoimmune; Experimental/immunology/*prevention & control, Flow Cytometry/methods, Gene Expression Regulation/immunology, Humans, Interferon-beta/*biosynthesis/genetics, Lectins; C-Type/genetics/metabolism, Lipopolysaccharides/pharmacology, Lymphocytes/immunology/metabolism, Major Histocompatibility Complex/genetics, Mice, Myelin-Associated Glycoprotein/immunology, Peptide Fragments/immunology, RNA; Messenger/biosynthesis, Rats, Rats; Inbred Lew, Receptors; Interleukin/genetics/metabolism, Receptors; Virus/genetics/metabolism, Research Support; Non-U.S. Gov't, Reverse Transcriptase Polymerase Chain Reaction/methods, Sequence Alignment, T-Lymphocytes/drug effects/immunology, Time Factors, Vaccination, Vaccines; DNA/*administration & dosage/immunology
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-70096DOI: 10.1016/j.jneuroim.2003.12.012PubMedID: 15020066OAI: oai:DiVA.org:uu-70096DiVA: diva2:98007
Available from: 2005-04-15 Created: 2005-04-15 Last updated: 2010-07-15Bibliographically approved

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Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=15020066&dopt=Citation

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