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Purification of the isoflavonoid puerarin by adsorption chromatography on cross-linked 12% agarose
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
2004 (English)In: J. Chromatogr. A, Vol. 1057, no 1-2, 95-100 p.Article in journal (Refereed) Published
Abstract [en]

The isoflavonoid puerarin in extracts of the well-known traditional Chinese drug Radix puerariae (root of the plant Pueraria lobata) can be separated from other isoflavonoids by adsorption chromatography on the cross-linked 12% agarose gel Superose 12 equilibrated in distilled water. The adsorption is totally quenched by the addition of 50% acetic acid. The separation of the isoflavonoids is tentatively ascribed to interaction with the residues of the cross-linking reagents used in the manufacturing process of Superose 12. Thus, no useful separation can be achieved with non-cross-linked 12% agarose gel media. Symmetric elution profiles at high sample loadings (16 mg on a 24 ml column) suggest linear adsorption isotherms for the isoflavonoids.

Place, publisher, year, edition, pages
2004. Vol. 1057, no 1-2, 95-100 p.
Keyword [en]
Isoflavonoid separation, Agarose cross-linker, Puerarin, Acetic acid quenching
Identifiers
URN: urn:nbn:se:uu:diva-70502OAI: oai:DiVA.org:uu-70502DiVA: diva2:98413
Available from: 2007-02-14 Created: 2007-02-14 Last updated: 2011-01-12

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