Automated solid-phase extraction method for the determination of piperaquine in capillary blood applied onto sampling paper by liquid chromatography
2004 (English)In: Journal of Chromatography B, no 809, 43-49 p.Article in journal (Refereed) Published
A bioanalytical method for the determination of piperaquine in 100 uL blood applied onto sampling paper, by solid-phase extraction and liquid chromatography, has been developed and validated. Blood spots were cut into small pieces prior to addition of 0.3 M perchloric acid, acetonitrile and phosphate buffer containing an internal standard. The liquid phase was loaded onto a mixed phase cation-exchange (MPC) solid-phase extraction column. Piperaquine and the internal standard were analysed by liquid chromatography and separated on a Chromolith Performance (100 mm x 4.6 mm) column with acetonitrile: phosphate buffer pH 2.5, I = 0.1 (8:92, v/v) at the flow of 3.5 mL/min. The UV detection was performed at 345 nm. the intra-assay precision was 12.0% at 0.150 uM, 7.3% at 1.25 uM and 7.3% at 2.25 uM. The inter-assay precison was 1.8% at 0.150 uM, 5.2% at 1.25 uM and 2.8% at 2.25 uM. The lower limit of quantification (LLOQ) was determined to 0.050 uM where the precison was 14.7%.
Place, publisher, year, edition, pages
2004. no 809, 43-49 p.
IdentifiersURN: urn:nbn:se:uu:diva-70705OAI: oai:DiVA.org:uu-70705DiVA: diva2:98616