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Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens
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2004 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 59, no 12, 1277-1284 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: No strawberry allergen has so far been identified and characterized.

METHODS: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.

RESULTS: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples, basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.

CONCLUSIONS: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.

Place, publisher, year, edition, pages
2004. Vol. 59, no 12, 1277-1284 p.
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-72000DOI: 10.1111/j.1398-9995.2004.00585.xPubMedID: 15507096OAI: oai:DiVA.org:uu-72000DiVA: diva2:99911
Available from: 2005-05-17 Created: 2005-05-17 Last updated: 2013-11-01Bibliographically approved

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Björk, Lars
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