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Cytokine-induced PGE2 formation is reduced from iNOS deficient murine islets
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2004 (English)In: Molecular and Cellular Endocrinology, ISSN 0303-7207, E-ISSN 1872-8057, Vol. 220, no 1-2, 21-29 p.Article in journal (Refereed) Published
Abstract [en]

Cytokines may be involved in islet destruction during Type 1 diabetes. Exposure to interleukin-1beta (IL-1beta) or IL-1beta plus interferon-gamma (IFN-gamma) of rodent islets induces expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Subsequent formation of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) may impair beta-cell function. Using iNOS deficient (iNOS -/-) islets, we have further investigated the relation between NO formation and PGE(2) induction. We found that iNOS -/- islets responded with a reduced PGE(2) formation following IL-1beta or (IL-1beta + IFN-gamma) treatment compared to wild-type (wt) islets, while COX-2 mRNA or protein content were unchanged. By the addition of an NO donor together with IL-1beta, PGE(2) formation could be stimulated from iNOS -/- islets. We conclude that the lowered capacity of PGE(2) formation observed from cytokine exposed iNOS -/- islets is due to a decreased stimulation of PGE(2) formation by the COX-2 enzyme in the absence of NO, rather then differences in expressed COX-2 protein.

Place, publisher, year, edition, pages
2004. Vol. 220, no 1-2, 21-29 p.
Keyword [en]
Islets of Langerhans, type 1 diabetes, interleukin-1β, nitric oxide, iNOS, prostaglandin E2
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-72025DOI: 10.1016/j.mce.2004.04.004ISI: 000222570100003PubMedID: 15196696OAI: oai:DiVA.org:uu-72025DiVA: diva2:99936
Available from: 2005-05-17 Created: 2005-05-17 Last updated: 2017-11-21Bibliographically approved

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Thorvaldson, LinaCarlsson, CarinaSandler, Stellan

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